The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4899-7708-3_18
Abstract: DNA strand breaks arise from normal cellular processes such as replication, transcription, and DNA repair as well as spontaneous DNA damage caused by cell metabolic activities. In addition, strand breaks occur due to direct or… read more here.
Keywords: random oligonucleotide; dna; strand breaks; dna strand ... See more keywords
Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6340-9_3
Abstract: Meiosis is a specialized form of cell division resulting in reproductive cells with a reduced, usually haploid, genome complement. A key step after premeiotic DNA replication is the occurrence of homologous recombination at multiple places… read more here.
Keywords: dna; sequencing spo11; dna double; strand breaks ... See more keywords
Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.06.009
Abstract: DNA single-strand breaks (SSBs) have attracted much interest since they are highly related to carcinogenesis and ageing. Herein, we report a new liquid crystal (LC)-based sensor for the detection of DNA SSBs generated by reactive… read more here.
Keywords: dna; dna single; liquid crystal; strand breaks ... See more keywords
Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity
Sign Up to like & getrecommendations! Published in 2022 at "iScience"
DOI: 10.1016/j.isci.2021.103621
Abstract: Summary Borna disease viruses (BoDV) have recently emerged as zoonotic neurotropic pathogens. These persistent RNA viruses assemble nuclear replication centers (vSPOT) in close interaction with the host chromatin. However, the topology of this interaction and… read more here.
Keywords: double strand; borna disease; dna double; strand breaks ... See more keywords
Necrosulfonamide Selectively Induces DNA Double-Strand Breaks in Acute Myeloid Leukemia Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical research in toxicology"
DOI: 10.1021/acs.chemrestox.2c00044
Abstract: Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy that causes endless pain for patients and accounts for thousands of deaths worldwide. The development of an effective AML treatment is a topic of ongoing interest.… read more here.
Keywords: acute myeloid; myeloid leukemia; strand breaks; double strand ... See more keywords
Radical Oxidation of the 2-Deoxyribosyl Moiety of DNA: Characterization of Unique Strand Breaks with 5'-Carboxylate Termini.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.5c00674
Abstract: Hydroxyl radicals and other reactive species attack DNA to produce an array of nucleobase and 2-deoxyribose lesions. Here, we have characterized novel oxidatively induced modifications of the sugar moiety that arise from initial H atom… read more here.
Keywords: radical oxidation; strand breaks; oxidation deoxyribosyl; moiety ... See more keywords
HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks
Sign Up to like & getrecommendations! Published in 2024 at "Cell Death Discovery"
DOI: 10.1038/s41420-024-02115-y
Abstract: Radiotherapy (RT) plays a critical role in the management of rhabdomyosarcoma (RMS), the prevalent soft tissue sarcoma in childhood. The high risk PAX3-FOXO1 fusion-positive subtype (FP-RMS) is often resistant to RT. We have recently demonstrated… read more here.
Keywords: dna double; inhibition; strand breaks; double strand ... See more keywords
DNA double-strand breaks in telophase lead to coalescence between segregated sister chromatid loci
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10742-8
Abstract: DNA double strand breaks (DSBs) pose a high risk for genome integrity. Cells repair DSBs through homologous recombination (HR) when a sister chromatid is available. HR is upregulated by the cycling dependent kinase (CDK) despite… read more here.
Keywords: sister chromatid; dna double; chromatid; strand breaks ... See more keywords
Dpb4 promotes resection of DNA double-strand breaks and checkpoint activation by acting in two different protein complexes
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-25090-9
Abstract: Budding yeast Dpb4 (POLE3/CHRAC17 in mammals) is a highly conserved histone fold protein that is shared by two protein complexes: the chromatin remodeler ISW2/hCHRAC and the DNA polymerase ε (Pol ε) holoenzyme. In Saccharomyces cerevisiae,… read more here.
Keywords: protein; dna; dna double; strand breaks ... See more keywords
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases
Sign Up to like & getrecommendations! Published in 2024 at "Nature Biomedical Engineering"
DOI: 10.1038/s41551-024-01277-5
Abstract: When used to edit genomes, Cas9 nucleases produce targeted double-strand breaks in DNA. Subsequent DNA-repair pathways can induce large genomic deletions (larger than 100 bp), which constrains the applicability of genome editing. Here we show that… read more here.
Keywords: dna deletions; base editors; repair; dna repair ... See more keywords
Dissociations of free radicals to generate protons, electrophiles or nucleophiles: role in DNA strand breaks.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Society reviews"
DOI: 10.1039/d1cs00193k
Abstract: The concept behind the research described in this article was that of marrying the 'soft' methods of radical generation with the effectiveness and flexibility of nucleophile/electrophile synthetic procedures. Classic studies with pulse radiolysis and laser… read more here.
Keywords: free radicals; dissociations free; radicals generate; dna ... See more keywords