The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4899-7708-3_18
Abstract: DNA strand breaks arise from normal cellular processes such as replication, transcription, and DNA repair as well as spontaneous DNA damage caused by cell metabolic activities. In addition, strand breaks occur due to direct or… read more here.
Keywords: random oligonucleotide; dna; strand breaks; dna strand ... See more keywords
Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6340-9_3
Abstract: Meiosis is a specialized form of cell division resulting in reproductive cells with a reduced, usually haploid, genome complement. A key step after premeiotic DNA replication is the occurrence of homologous recombination at multiple places… read more here.
Keywords: dna; sequencing spo11; dna double; strand breaks ... See more keywords
Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.06.009
Abstract: DNA single-strand breaks (SSBs) have attracted much interest since they are highly related to carcinogenesis and ageing. Herein, we report a new liquid crystal (LC)-based sensor for the detection of DNA SSBs generated by reactive… read more here.
Keywords: dna; dna single; liquid crystal; strand breaks ... See more keywords
Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity
Sign Up to like & getrecommendations! Published in 2022 at "iScience"
DOI: 10.1016/j.isci.2021.103621
Abstract: Summary Borna disease viruses (BoDV) have recently emerged as zoonotic neurotropic pathogens. These persistent RNA viruses assemble nuclear replication centers (vSPOT) in close interaction with the host chromatin. However, the topology of this interaction and… read more here.
Keywords: double strand; borna disease; dna double; strand breaks ... See more keywords
Necrosulfonamide Selectively Induces DNA Double-Strand Breaks in Acute Myeloid Leukemia Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical research in toxicology"
DOI: 10.1021/acs.chemrestox.2c00044
Abstract: Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy that causes endless pain for patients and accounts for thousands of deaths worldwide. The development of an effective AML treatment is a topic of ongoing interest.… read more here.
Keywords: acute myeloid; myeloid leukemia; strand breaks; double strand ... See more keywords
DNA double-strand breaks in telophase lead to coalescence between segregated sister chromatid loci
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10742-8
Abstract: DNA double strand breaks (DSBs) pose a high risk for genome integrity. Cells repair DSBs through homologous recombination (HR) when a sister chromatid is available. HR is upregulated by the cycling dependent kinase (CDK) despite… read more here.
Keywords: sister chromatid; dna double; chromatid; strand breaks ... See more keywords
Dpb4 promotes resection of DNA double-strand breaks and checkpoint activation by acting in two different protein complexes
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-25090-9
Abstract: Budding yeast Dpb4 (POLE3/CHRAC17 in mammals) is a highly conserved histone fold protein that is shared by two protein complexes: the chromatin remodeler ISW2/hCHRAC and the DNA polymerase ε (Pol ε) holoenzyme. In Saccharomyces cerevisiae,… read more here.
Keywords: protein; dna; dna double; strand breaks ... See more keywords
Dissociations of free radicals to generate protons, electrophiles or nucleophiles: role in DNA strand breaks.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Society reviews"
DOI: 10.1039/d1cs00193k
Abstract: The concept behind the research described in this article was that of marrying the 'soft' methods of radical generation with the effectiveness and flexibility of nucleophile/electrophile synthetic procedures. Classic studies with pulse radiolysis and laser… read more here.
Keywords: free radicals; dissociations free; radicals generate; dna ... See more keywords
Deciphering the functional mechanism of zinc ions of PARP1 binding with single strand breaks and double strand breaks
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra02683j
Abstract: Poly(ADP-ribose)polymerase 1 (PARP1) is a key target for the treatment of cancer-related diseases, and plays an important role in biological processes such as DNA repair, regulating a variety of metabolic and signal transduction processes. Understanding… read more here.
Keywords: znf1 znf2; strand breaks; dna; zinc ions ... See more keywords
Single-molecule force spectroscopy reveals binding and bridging dynamics of PARP1 and PARP2 at DNA double-strand breaks
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2214209120
Abstract: Significance PARP [Poly(ADP-ribose) polymerase] proteins are important signaling enzymes in eukaryotic DNA damage repair. We investigated how PARP1 and PARP2 bind at DNA double-strand breaks (DSBs) by developing a single-molecule magnetic tweezers assay to measure… read more here.
Keywords: dna; parp1 parp2; spectroscopy; dna double ... See more keywords
How to explain the sensitivity of DNA double-strand breaks yield to 125I position?
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Radiation Biology"
DOI: 10.1080/09553002.2022.2047822
Abstract: Abstract Purpose Auger emitters exhibit interesting features due to their emission of a cascade of short-range Auger electrons. Maximum DNA breakage efficacy is achieved when decays occur near DNA. Studies of double-strand breaks (DSBs) yields… read more here.
Keywords: dna; strand breaks; 125i position; double strand ... See more keywords