A strand displacement signal amplification-assisted fluorescence assay for sensitive detection of microRNA.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of separation science"
DOI: 10.1002/jssc.202300123
Abstract: MicroRNA is a vital biomarker because of its abnormal expression in the emergence and development of diseases, especially in cancers. Herein, a label-free fluorescent sensing platform is proposed for detecting microRNA-21, coupled with the cascade… read more here.
Keywords: signal amplification; amplification assisted; displacement signal; detection ... See more keywords
DNA Kirigami Driven by Polymerase-Triggered Strand Displacement.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202201478
Abstract: The precursors of functional biomolecules in living cells are synthesized in a bottom-up manner and subsequently activated by modification into a delicate structure with near-atomic precision. DNA origami technology provides a promising way to mimic… read more here.
Keywords: polymerase triggered; strand displacement; dna kirigami; dna ... See more keywords
Ultrasensitive assay based on a combined cascade amplification by nicking-mediated rolling circle amplification and symmetric strand-displacement amplification.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.10.004
Abstract: MicroRNAs (miRNAs) play an important role in the regulation of various biological processes and have been used as potential biomarkers for biomedical research and clinical diagnosis. Here, nicking-mediated rolling circle amplification (N-RCA)/symmetric isothermal circular strand-displacement… read more here.
Keywords: strand displacement; rolling circle; nicking mediated; circle amplification ... See more keywords
Hydrazone chemistry mediated toehold strand displacement cascade and its application for 5-hydroxymethylfurfural analysis.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.01.008
Abstract: Hydrazone chemistry has been firstly explored as capturing mode for interface supported toehold strand displacement cascade (TSDC). The method has been further established for analysis of 5-hydroxymethylfurfural (HMF) based on hydrazone chemistry-mediated TSDC. HMF containing… read more here.
Keywords: strand displacement; chemistry; hydrazone chemistry; displacement cascade ... See more keywords
Strand-displacement DNA polymerase induced isothermal circular amplification fluorescence sensor for identification of pork component
Sign Up to like & getrecommendations! Published in 2021 at "Food bioscience"
DOI: 10.1016/j.fbio.2021.101189
Abstract: Abstract Proper labeling of meat products is critical for consumers who want to avoid specific meat species for cultural or religious reasons. Herein, we developed a convenient strand-displacement DNA polymerase induced isothermal circular amplification (SDPICA)… read more here.
Keywords: component; pork; strand displacement; meat ... See more keywords
Cascade strand displacement reaction-assisted aptamer-based highly sensitive detection of ochratoxin A.
Sign Up to like & getrecommendations! Published in 2020 at "Food chemistry"
DOI: 10.1016/j.foodchem.2020.127827
Abstract: Ochratoxin A (OTA) is a toxic metabolite that is widely distributed in food products. Herein, we proposed a new fluorescent aptasensor for OTA detection by using cascade strand displacement reaction. The binding of OTA and… read more here.
Keywords: displacement reaction; strand displacement; detection;
Novel RNase H inhibitors blocking RNA-directed strand displacement DNA synthesis by HIV-1 reverse transcriptase.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2022.167507
Abstract: In retroviruses, strand displacement DNA-dependent DNA polymerization catalyzed by the viral reverse transcriptase (RT) is required to synthesize double-stranded proviral DNA. In addition, strand displacement during RNA-dependent DNA synthesis is critical to generate high-quality cDNA… read more here.
Keywords: dna; strand displacement; rnase; displacement dna ... See more keywords
Rolling-Circle and Strand-displacement Mechanisms for Non-enzymatic RNA Replication at the Time of the Origin of Life.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of theoretical biology"
DOI: 10.1016/j.jtbi.2021.110822
Abstract: It is likely that RNA replication began non-enzymatically, and that polymerases were later selected to speed up the process. We consider replication mechanisms in modern viruses and ask which of these is possible non-enzymatically, using… read more here.
Keywords: strand displacement; rolling circle; rna; strand ... See more keywords
A cascade strand displacement amplification strategy for highly sensitive and label-free detection of DNA methyltransferase activity
Sign Up to like & getrecommendations! Published in 2021 at "Microchemical Journal"
DOI: 10.1016/j.microc.2020.105775
Abstract: Abstract DNA methyltransferase (MTase) is regarded as a promising biomarker and therapeutic target, which plays an important regulatory role in many biological processes. Here, a cascade strand displacement amplification strategy was explored for highly sensitive… read more here.
Keywords: strand displacement; detection; dna; displacement amplification ... See more keywords
Intermolecular and Intramolecular Priming Co-directed Synergistic Multi-strand Displacement Amplification Empowers Ultrasensitive Determination of microRNAs.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03466
Abstract: The construction of isothermal nucleic acid amplification systems with extremely high signal amplification abilities is of great importance for biomarker detection and disease diagnosis. Herein, for the first time, we proposed an intermolecular and intramolecular… read more here.
Keywords: intramolecular priming; amplification; strand displacement; priming directed ... See more keywords
Single Primer Based Multisite Strand Displacement Reaction Amplification Strategy for Rapid Detection of Terminal Deoxynucleotidyl Transferase Activity.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b01816
Abstract: A fluorescence-based multisite strand displacement reaction (MSSDR) amplification strategy is developed for the rapid, sensitive, and selective detection the activity of terminal deoxynucleotidyl transferase (TdT). Oligo dT primer was used for the TdT extension reaction,… read more here.
Keywords: strand displacement; detection; based multisite; reaction ... See more keywords