Strand-Specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding Yeast.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7306-4_17
Abstract: DNA replication initiates at DNA replication origins after unwinding of double-strand DNA(dsDNA) by replicative helicase to generate single-stranded DNA (ssDNA) templates for the continuous synthesis of leading-strand and the discontinuous synthesis of lagging-strand. Therefore, methods… read more here.
Keywords: strand specific; dna; dna replication; strand ... See more keywords
Characterization of TK1646, a highly thermostable 3'-5' single strand specific exonuclease from Thermococcus kodakarensis.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.08.150
Abstract: Exonucleases catalyze the hydrolysis of terminal phosphodiester bond in nucleic acid. They play important role in maintaining the integrity of DNA in eukaryotes, prokaryotes and archaea. Limited studies have been done on archaeal exonucleases. Here… read more here.
Keywords: strand specific; single strand; exonuclease; specific exonuclease ... See more keywords
Long non-coding and coding RNA profiling using strand-specific RNA-seq in human hypertrophic cardiomyopathy
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Data"
DOI: 10.1038/s41597-019-0094-6
Abstract: Hypertrophic cardiomyopathy (HCM) represents one of the most common heritable heart diseases. However, the signalling pathways and regulatory networks underlying the pathogenesis of HCM remain largely unknown. Here, we present a strand-specific RNA-seq dataset for… read more here.
Keywords: specific rna; strand specific; rna; hypertrophic cardiomyopathy ... See more keywords
Genome-wide Identification and Characterization of Natural Antisense Transcripts by Strand-specific RNA Sequencing in Ganoderma lucidum
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-04303-6
Abstract: Ganoderma lucidum is a white-rot fungus best-known for its medicinal and ligninolytic activities. To discover the underlying genes responsible for these activities, we identified and characterized the natural antisense transcripts (NATs) using strand-specific (ss) RNA-seq… read more here.
Keywords: strand specific; rna; natural antisense; expression ... See more keywords
Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing.
Sign Up to like & getrecommendations! Published in 2023 at "Genome research"
DOI: 10.1101/gr.277213.122
Abstract: Tn5 transposon tagments double-stranded DNA and RNA/DNA hybrids to generate nucleic acids that are ready to be amplified for high-throughput sequencing. The nucleic acid substrates for the Tn5 transposon must be explored to increase the… read more here.
Keywords: single stranded; dna; rna; strand specific ... See more keywords
Reinterpreting sheep muscle strand-specific RNA sequencing data showing extensive 3'UTR extensions.
Sign Up to like & getrecommendations! Published in 2020 at "Animal genetics"
DOI: 10.1111/age.12987
Abstract: The more complex 3' UTR in higher organisms may have the function of increasing post-transcriptional gene regulation. Recent RNA sequencing technologies have provided us with the possibility to capture the complete 3' UTR landscape of… read more here.
Keywords: rna sequencing; strand specific; sheep muscle; utr extensions ... See more keywords
Genome-wide analysis of rice cis-natural antisense transcription under cadmium exposure using strand-specific RNA-Seq
Sign Up to like & getrecommendations! Published in 2017 at "BMC Genomics"
DOI: 10.1186/s12864-017-4108-5
Abstract: BackgroundThe elucidation of novel transcripts and their expression in response to various stress conditions is necessary to understand the transcriptional network of plants as an adaptation to biotic and abiotic stresses. We performed strand-specific RNA-Seq… read more here.
Keywords: cis nats; strand specific; exposure; rap transcripts ... See more keywords
The Identification of MATE Antisense Transcripts in Soybean Using Strand-Specific RNA-Seq Datasets
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13020228
Abstract: Natural antisense transcripts (NATs) have been generally reported as negative regulators of their sense counterparts. Multidrug and toxic compound extrusion (MATE) proteins mediate the transport of various substrates. Although MATEs have been identified genome-wide in… read more here.
Keywords: antisense transcripts; specific rna; mate antisense; mate ... See more keywords