Recent advances in the synthesis of single‐stranded DNA in vitro
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202400026
Abstract: Single‐stranded DNA (ssDNA) is the foundation of modern biology, with wide applications in gene editing, sequencing, DNA information storage, and materials science. However, synthesizing ssDNA with high efficiency, high throughput, and low error rate in… read more here.
Keywords: stranded dna; vitro; synthesis; ssdna synthesis ... See more keywords
G‐quadruplex‐enhanced circular single‐stranded DNA (G4‐CSSD) adsorption of miRNA to inhibit colon cancer progression
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Medicine"
DOI: 10.1002/cam4.5721
Abstract: Chromosomal heterogeneity leads to the abnormal expression and mutation of tumor‐specific genes. Drugs targeting oncogenes have been extensively developed. However, given the random mutation of tumor suppressor genes, the development of its targeted drugs is… read more here.
Keywords: single stranded; quadruplex enhanced; stranded dna; enhanced circular ... See more keywords
Spacer‐Complementary Single‐Stranded DNA Oligonucleotides Can Serve as Target‐Specific Inhibitors in CRISPR/Cas9 Systems
Sign Up to like & getrecommendations! Published in 2025 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.70088
Abstract: The continuous expression of the CRISPR/Cas system in organisms can lead to various potential issues. Some anti‐CRISPR strategies have been developed to achieve precise control over CRISPR/Cas, yet these strategies are predominantly protein‐based, with the… read more here.
Keywords: stranded dna; target; crispr; target specific ... See more keywords
Investigation on the Peroxidase‐Like Activity of Co3O4 Nanoparticles Adsorbed with Diverse DNA
Sign Up to like & getrecommendations! Published in 2025 at "ChemBioChem"
DOI: 10.1002/cbic.202500579
Abstract: In recent years, nanozymes have increasingly attracted the attention of researchers due to their low cost, high stability, and straightforward preparation when compared with natural enzymes. Furthermore, the biocompatibility of nanozymes has been enhanced through… read more here.
Keywords: stranded dna; co3o4 nanozyme; diverse dna; co3o4 ... See more keywords
RAG1/2 induces double‐stranded DNA breaks at non‐Ig loci in the proximity of single sequence repeats in developing B cells
Sign Up to like & getrecommendations! Published in 2024 at "European Journal of Immunology"
DOI: 10.1002/eji.202350958
Abstract: In developing B cells, V(D)J gene recombination is initiated by the RAG1/2 endonuclease complex, introducing double‐stranded DNA breaks (DSBs) in V, D, and J genes and resulting in the formation of the hypervariable parts of… read more here.
Keywords: stranded dna; double stranded; developing cells; rag1 ... See more keywords
An Efficient Bead‐captured Denaturation Method for Preparing Long Single‐stranded DNA
Sign Up to like & getrecommendations! Published in 2017 at "Journal of The Chinese Chemical Society"
DOI: 10.1002/jccs.201700142
Abstract: Previous methods to prepare single-stranded DNA (ssDNA) substrates are limited to short DNA lengths and inefficient. We have developed an efficient and rapid method to prepare long ssDNA substrates (up to 4000 nt) based on the… read more here.
Keywords: single stranded; dna; ssdna; bead captured ... See more keywords
The intrinsically disordered linker of E. coli SSB is critical for the release from single‐stranded DNA
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3115
Abstract: The Escherichia coli single stranded DNA binding protein (SSB) is crucial for DNA replication, recombination and repair. Within each process, it has two seemingly disparate roles: it stabilizes single‐stranded DNA (ssDNA) intermediates generated during DNA… read more here.
Keywords: single stranded; dna; ssb; stranded dna ... See more keywords
Two different kinds of interaction modes of deaminase APOBEC3A with single‐stranded DNA in solution detected by nuclear magnetic resonance
Sign Up to like & getrecommendations! Published in 2021 at "Protein Science"
DOI: 10.1002/pro.4242
Abstract: APOBEC3A (A3A) deaminates deoxycytidine in target motif TC in a single‐stranded DNA (we termed it as TC DNA), which mortally mutates viral pathogens and immunoglobulins, and leads to the diversification and lethality of cancers. The… read more here.
Keywords: dna; stranded dna; two different; solution ... See more keywords
Single-Stranded DNA-Binding Proteins in the Archaea.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_2
Abstract: Single-stranded (ss) DNA-binding proteins are found in all three domains of life where they play vital roles in nearly all aspects of DNA metabolism by binding to and stabilizing exposed ssDNA and acting as platforms… read more here.
Keywords: single stranded; ssdna binding; binding; stranded dna ... See more keywords
Measuring the Complex Effects of the Single-Stranded DNA-Binding Protein gp2.5 on Primer Synthesis and Extension by the Bacteriophage T7 Replisome.
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DOI: 10.1007/978-1-0716-1290-3_21
Abstract: The single-stranded DNA-binding protein gp2.5 of bacteriophage T7 plays myriad functions in the replication of phage genomes. In addition to interacting with ssDNA, gp2.5 binds to the T7 DNA polymerase and primase/helicase proteins, regulating their… read more here.
Keywords: single stranded; gp2 primer; protein gp2; stranded dna ... See more keywords
Structural Characterization of a Single-Stranded DNA-Binding Protein: A Case Study of the ORF6 Protein from Bacteriophage Enc34.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_23
Abstract: In the quest to understand how single-stranded DNA-binding proteins function and evolve at a molecular level, determination of their high-resolution three-dimensional structure using methods such as X-ray crystallography is indispensable. Here we present a collection… read more here.
Keywords: single stranded; biology; stranded dna; binding protein ... See more keywords