Dissipation of double-stranded RNA in aquatic microcosms.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental toxicology and chemistry"
DOI: 10.1002/etc.3648
Abstract: Silencing genes of a pest with double-stranded RNA (dsRNA) is a promising new pest management technology. As part of the environmental risk assessment for dsRNA-based products, the environmental fate and the potential for adverse effects… read more here.
Keywords: dissipation double; water; double stranded; stranded rna ... See more keywords
Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0138-9_14
Abstract: This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing… read more here.
Keywords: rna viruses; double stranded; stranded rna; throughput sequencing ... See more keywords
Double-Stranded RNA-Mediated Suppression of Trypsin-Like Serine Protease (t-SP) Triggers Over-Expression of Another t-SP Isoform in Helicoverpa armigera
Sign Up to like & getrecommendations! Published in 2017 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-017-2584-3
Abstract: High diversity of digestive proteases is considered to be the key factor in the evolution of polyphagy in Helicoverpa armigera. Serine proteases (SPs) contribute ~85% of the dietary protein digestion in H. armigera. We investigated… read more here.
Keywords: helicoverpa armigera; isoform; double stranded; stranded rna ... See more keywords
Micropeptide vsp21 translated by Reovirus circular RNA 000048 attenuates viral replication.
Sign Up to like & getrecommendations! Published in 2022 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2022.04.136
Abstract: To date, some DNA viruses and single-stranded RNA viruses have been found to generate circRNAs. However, the reports on circRNAs produced by double-stranded RNA viruses are very limited. In this study, Bombyx mori cypovirus (BmCPV),… read more here.
Keywords: attenuates viral; viral replication; micropeptide vsp21; stranded rna ... See more keywords
Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum.
Sign Up to like & getrecommendations! Published in 2018 at "Virology"
DOI: 10.1016/j.virol.2018.03.008
Abstract: Here we describe a novel (-)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs… read more here.
Keywords: mycovirus; fusarium graminearum; negative stranded; stranded rna ... See more keywords
A dsRNA virus with filamentous viral particles
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-00237-9
Abstract: Viruses with double-stranded RNA genomes form isometric particles or are capsidless. Here we report a double-stranded RNA virus, Colletotrichum camelliae filamentous virus 1 (CcFV-1) isolated from a fungal pathogen, that forms filamentous particles. CcFV-1 has… read more here.
Keywords: double stranded; stranded rna; virus filamentous; filamentous particles ... See more keywords
Effective inactivated influenza vaccine for the elderly using a single-stranded RNA-based adjuvant
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-91445-3
Abstract: There is an unmet need for new influenza vaccine strategies that compensate for impaired vaccine responses in elderly individuals. Here, we evaluated the effectiveness of a single-stranded RNA (ssRNA) as an adjuvant to enhance the… read more here.
Keywords: single stranded; influenza vaccine; stranded rna; inactivated influenza ... See more keywords
Sequence-dependent mechanical properties of double-stranded RNA.
Sign Up to like & getrecommendations! Published in 2019 at "Nanoscale"
DOI: 10.1039/c9nr07516j
Abstract: The mechanical properties of double-stranded RNA (dsRNA) are involved in many of its biological functions and are relevant for future nanotechnology applications. DsRNA must tightly bend to fit inside viral capsids or deform upon the… read more here.
Keywords: properties double; response; double stranded; stranded rna ... See more keywords
Transcriptome-wide identification of single-stranded RNA binding proteins
Sign Up to like & getrecommendations! Published in 2023 at "Chemical Science"
DOI: 10.1039/d3sc00957b
Abstract: RNA–protein interactions are precisely regulated by RNA secondary structures in various biological processes. Large-scale identification of proteins that interact with particular RNA structure is important to the RBPome. Herein, a kethoxal assisted single-stranded RNA interactome… read more here.
Keywords: rna binding; single stranded; identification; stranded rna ... See more keywords
Double-stranded RNA immunohistochemistry as a screening tool for viral encephalitis.
Sign Up to like & getrecommendations! Published in 2023 at "American journal of clinical pathology"
DOI: 10.1093/ajcp/aqad039
Abstract: OBJECTIVES Viral infections of the central nervous system can be challenging to diagnose because of the wide range of causative agents and nonspecific histologic features. We sought to determine whether detection of double-stranded RNA (dsRNA),… read more here.
Keywords: double stranded; immunohistochemistry; viral infections; stranded rna ... See more keywords
Compositional complementarity between genomic RNA and coat proteins in positive-sense single-stranded RNA viruses
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac202
Abstract: Abstract During packaging in positive-sense single-stranded RNA (+ssRNA) viruses, coat proteins (CPs) interact directly with multiple regions in genomic RNA (gRNA), but the underlying physicochemical principles remain unclear. Here we analyze the high-resolution cryo-EM structure… read more here.
Keywords: single stranded; coat proteins; genomic rna; stranded rna ... See more keywords