Target‐Catalyzed Self‐Assembly of DNA‐Streptavidin Nanogel for Enzyme‐Free miRNA Assay
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202202076
Abstract: Rapid, sensitive, specific, and user‐friendly microRNA (miRNA) assays are in high demand for point‐of‐care diagnosis. Target‐catalyzed toehold‐mediated strand displacement (TMSD) has received increasing attention as an enzyme‐free molecular tool for DNA detection. However, the application… read more here.
Keywords: target catalyzed; dna; enzyme free; mirna assay ... See more keywords
Refolding with Simultaneous Purification of Recombinant Core Streptavidin Using Single-step High-performance Hydrophobic Interaction Chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-019-0041-6
Abstract: Streptavidin has applied to many areas including detection, purification, labeling, crosslinking and immobilization resulting in a high demand on its production. In this study, we report a method for preparation of recombinant core streptavidin (cSAV)… read more here.
Keywords: chromatography; hydrophobic interaction; recombinant core; streptavidin ... See more keywords
Chemical Derivatization of Affinity Matrices Provides Protection from Tryptic Proteolysis.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00254
Abstract: The enrichment of biotinylated proteins using immobilized streptavidin has become a staple methodology for affinity purification-based proteomics. Many of these workflows rely upon tryptic digestion to elute streptavidin-captured moieties from the beads. The concurrent release… read more here.
Keywords: affinity matrices; proteolysis; affinity; streptavidin ... See more keywords
Electrochemical Microfluidic Paper-Based Aptasensor Platform Based on a Biotin-Streptavidin System for Label-Free Detection of Biomarkers.
Sign Up to like & getrecommendations! Published in 2021 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.1c12716
Abstract: Timely and rapid detection of biomarkers is extremely important for the diagnosis and treatment of diseases. However, going to the hospital to test biomarkers is the most common way. People need to spend a lot… read more here.
Keywords: detection; system; paper; streptavidin ... See more keywords
Antifungal activity of streptavidin C1 and C2 against pathogens causing Fusarium wilt
Sign Up to like & getrecommendations! Published in 2021 at "Letters in Applied Microbiology"
DOI: 10.1111/lam.13533
Abstract: Fusarium wilt is caused by the soil‐inhabiting fungus Fusarium oxysporum ff. spp. and is one of the most devastating plant diseases, resulting in losses and decreasing the quality and safety of agricultural crops. We recently… read more here.
Keywords: fusarium; fusarium wilt; streptavidin; soil ... See more keywords
Production of mutant streptavidin protein and investigation of its effect on the performance of streptavidin
Sign Up to like & getrecommendations! Published in 2023 at "Iranian Journal of Basic Medical Sciences"
DOI: 10.22038/ijbms.2023.69083.15060
Abstract: Objective(s): Streptavidin is a versatile protein in cell science. The tetramer structure of streptavidin plays a key role in this binding, but this form interferes with some assays. If monomer streptavidin is still capable of… read more here.
Keywords: production mutant; effect; mutant streptavidin; biotin ... See more keywords
Immobilization of Streptavidin on a Plasmonic Au-TiO2 Thin Film towards an LSPR Biosensing Platform
Sign Up to like & getrecommendations! Published in 2022 at "Nanomaterials"
DOI: 10.3390/nano12091526
Abstract: Optical biosensors based on localized surface plasmon resonance (LSPR) are the future of label-free detection methods. This work reports the development of plasmonic thin films, containing Au nanoparticles dispersed in a TiO2 matrix, as platforms… read more here.
Keywords: platform; tio2 thin; tio2; streptavidin ... See more keywords
Biotin Interference in Susceptible CanAg Ca242 Immunoassays and Elimination Method.
Sign Up to like & getrecommendations! Published in 2020 at "Clinical laboratory"
DOI: 10.7754/clin.lab.2019.191141
Abstract: BACKGROUND High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS). Biotin, as dietary supplement or therapy, may lead to analytical interference in biotin-streptavidin immunoassay. METHODS Seven concentration gradients of biotin solutions were spiked to… read more here.
Keywords: biotin interference; biotin; streptavidin; canag ca242 ... See more keywords