Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production.
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DOI: 10.1007/978-1-4939-6691-2_8
Abstract: The choice of an expression system for the metagenomic DNA of interest is of vital importance for the detection of any particular gene or gene cluster. Most of the screens to date have used the… read more here.
Keywords: production; metagenomic dna; dna; streptomyces lividans ... See more keywords
Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
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DOI: 10.1186/s12934-018-1040-6
Abstract: BackgroundThe Gram-positive Streptomyces lividans TK24 is an attractive host for heterologous protein production because of its high capability to secrete proteins—which favors correct folding and facilitates downstream processing—as well as its acceptance of methylated DNA… read more here.
Keywords: protein; protein production; streptomyces lividans; expression ... See more keywords
Dynamics of Pellet Fragmentation and Aggregation in Liquid-Grown Cultures of Streptomyces lividans
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DOI: 10.3389/fmicb.2018.00943
Abstract: Streptomycetes are extensively used for the production of valuable products, including various antibiotics and industrial enzymes. The preferred way to grow these bacteria in industrial settings is in large-scale fermenters. Growth of streptomycetes under these… read more here.
Keywords: aggregation liquid; pellet fragmentation; dynamics pellet; fragmentation aggregation ... See more keywords
Characterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions
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DOI: 10.3389/fmicb.2018.03033
Abstract: Alternative sigma factors control numerous aspects of bacterial life, including adaptation to physiological stresses, morphological development, persistence states and virulence. This is especially true for the physiologically complex actinobacteria. Here we report the development of… read more here.
Keywords: gene deletions; lividans tk24; streptomyces lividans; gene ... See more keywords
Engineered Streptomyces lividans Strains for Optimal Identification and Expression of Cryptic Biosynthetic Gene Clusters
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DOI: 10.3389/fmicb.2018.03042
Abstract: Streptomyces lividans is a suitable host for the heterologous expression of biosynthetic gene clusters (BGCs) from actinomycetes to discover “cryptic” secondary metabolites. To improve the heterologous expression of BGCs, herein we optimized S. lividans strain… read more here.
Keywords: production; streptomyces lividans; expression; host ... See more keywords
The Transcription Unit Architecture of Streptomyces lividans TK24
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DOI: 10.3389/fmicb.2019.02074
Abstract: Streptomyces lividans is an attractive host for production of heterologous proteins and secondary metabolites of other Streptomyces species. To fully harness the industrial potential of S. lividans, understanding its metabolism and genetic regulatory elements is… read more here.
Keywords: transcription unit; transcription; streptomyces lividans; unit architecture ... See more keywords
The Generation of an Artificial ATP Deficit Triggers Antibiotic Production in Streptomyces lividans
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DOI: 10.3390/antibiotics11091157
Abstract: In most Streptomyces species, antibiotic production is triggered in a condition of phosphate limitation, a condition that is known to be correlated with a low intracellular ATP content compared to growth in a condition of… read more here.
Keywords: antibiotic production; atp deficit; functional atpase; production ... See more keywords