Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR‐Cas9 Activation
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800266
Abstract: Silent biosynthetic gene clusters represent a potentially rich source of new bioactive compounds. We report the discovery, characterization, and biosynthesis of a novel doubly glycosylated 24‐membered polyene macrolactam from a silent biosynthetic gene cluster in… read more here.
Keywords: crispr cas9; gene cluster; streptomyces roseosporus; activation ... See more keywords
Degradation mechanism of AtrA mediated by ClpXP and its application in daptomycin production in Streptomyces roseosporus
Sign Up to like & getrecommendations! Published in 2023 at "Protein Science"
DOI: 10.1002/pro.4617
Abstract: The efficiency of drug biosynthesis depends on different transcriptional regulatory pathways in Streptomyces, and the protein degradation system adds another layer of complexity to the regulatory processes. AtrA, a transcriptional regulator in the A‐factor regulatory… read more here.
Keywords: mechanism atra; daptomycin; production; degradation mechanism ... See more keywords
Crotonylation of key metabolic enzymes regulates carbon catabolite repression in Streptomyces roseosporus
Sign Up to like & getrecommendations! Published in 2020 at "Communications Biology"
DOI: 10.1038/s42003-020-0924-2
Abstract: Due to the plethora natural products made by Streptomyces, the regulation of its metabolism are of great interest, whereas there is a lack of detailed understanding of the role of posttranslational modifications (PTM) beyond traditional… read more here.
Keywords: metabolism; catabolite repression; carbon catabolite; streptomyces roseosporus ... See more keywords
Roseosporol A, the first isolation of a novel sesquiterpenoid from Streptomyces roseosporus
Sign Up to like & getrecommendations! Published in 2019 at "Natural Product Research"
DOI: 10.1080/14786419.2018.1484457
Abstract: Abstract A novel sesquiterpenoid, roseosporol A (1), together with 12 known compounds (2–13) were isolated from the ethyl acetate extract of Lsr2-deletion mutant strain of Streptomyces roseosporus. Their structures were determined by spectroscopic methods, including… read more here.
Keywords: novel sesquiterpenoid; isolation novel; roseosporol first; streptomyces roseosporus ... See more keywords
Improving the Yield and Quality of Daptomycin in Streptomyces roseosporus by Multilevel Metabolic Engineering
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.872397
Abstract: Daptomycin is a cyclic lipopeptide antibiotic with a significant antibacterial action against antibiotic-resistant Gram-positive bacteria. Despite numerous attempts to enhance daptomycin yield throughout the years, the production remains unsatisfactory. This study reports the application of… read more here.
Keywords: multilevel metabolic; metabolic engineering; quality daptomycin; streptomyces roseosporus ... See more keywords
Transcriptional Regulator DasR Represses Daptomycin Production through Both Direct and Cascade Mechanisms in Streptomyces roseosporus
Sign Up to like & getrecommendations! Published in 2022 at "Antibiotics"
DOI: 10.3390/antibiotics11081065
Abstract: Daptomycin, produced by Streptomyces roseosporus, is a clinically important cyclic lipopeptide antibiotic used for the treatment of human infections caused by drug-resistant Gram-positive pathogens. In contrast to most Streptomyces antibiotic biosynthetic gene clusters (BGCs), daptomycin… read more here.
Keywords: production; daptomycin production; streptomyces roseosporus; dasr ... See more keywords