Quantification of the soluble Receptor of Advanced Glycation End-Products (sRAGE) by LC-MS after enrichment by strong cation exchange (SCX) solid-phase extraction (SPE) at the protein level.
Sign Up to like & getrecommendations! Published in 2018 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.09.050
Abstract: The study of low abundant proteins contributes to increasing our knowledge about (patho)physiological processes and may lead to the identification and clinical application of disease markers. However, studying these proteins is challenging as high-abundant proteins… read more here.
Keywords: cation exchange; enrichment; exchange scx; strong cation ... See more keywords
Basic Strong Cation Exchange Chromatography, BaSCX, a Highly Efficient Approach for C-Terminomic Studies Using LysargiNase Digestion.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b05280
Abstract: Decoding protein C-termini is a challenging task in protein chemistry using conventional chemical/enzymatic approaches. With the rapid development in modern mass spectrometer, many advanced mass spectrometry (MS) based protein C-termini analysis approaches have been established.… read more here.
Keywords: protein; cation exchange; protein termini; chemistry ... See more keywords