MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-45827-6
Abstract: Analyzing immune cell interactions in the bone marrow is vital for understanding hematopoiesis and bone homeostasis. Three-dimensional analysis of the complete, intact bone marrow within the cortex of whole long bones remains a challenge, especially… read more here.
Keywords: bone marrow; analysis; bone; subcellular resolution ... See more keywords
Computationally designed sensors detect endogenous Ras activity and signaling effectors at subcellular resolution
Sign Up to like & getrecommendations! Published in 2024 at "Nature Biotechnology"
DOI: 10.1038/s41587-023-02107-w
Abstract: The utility of genetically encoded biosensors for sensing the activity of signaling proteins has been hampered by a lack of strategies for matching sensor sensitivity to the physiological concentration range of the target. Here we… read more here.
Keywords: endogenous ras; ras activity; activity signaling; subcellular resolution ... See more keywords
Application of the Mesolens for subcellular resolution imaging of intact larval and whole adult Drosophila
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Microscopy"
DOI: 10.1111/jmi.12693
Abstract: In a previous paper, we showed a new giant lens called the Mesolens and presented performance data and images from whole fixed and intact fluorescently‐stained 12.5‐day old mouse embryos. Here, we show that using the… read more here.
Keywords: drosophila; resolution imaging; adult; mesolens subcellular ... See more keywords
Abstract 121: In situ mutational mapping of tumor cells and tissues at subcellular resolution
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-121
Abstract: In situ techniques enable studying cells and tissues within their cellular context. This is of great importance in cancer, often displaying genetic and phenotypic heterogeneity with distinct subclones harboring different capacity to grow and spread.… read more here.
Keywords: mutational mapping; tumor; situ mutational; cells tissues ... See more keywords
Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation.
Sign Up to like & getrecommendations! Published in 2019 at "Optics letters"
DOI: 10.1364/ol.44.003166
Abstract: We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent… read more here.
Keywords: astrocyte imaging; harmonic generation; third harmonic; stain free ... See more keywords