Improving the catalytic efficiency and substrate affinity of a novel esterase from marine Klebsiella aerogenes by random and site-directed mutation
Sign Up to like & getrecommendations! Published in 2021 at "World Journal of Microbiology and Biotechnology"
DOI: 10.1007/s11274-021-03069-4
Abstract: A novel esterase ( Est Ka) from marine Klebsiella aerogenes was characterized with hydrolytic activity against p -nitrophenyl caprylate ( p NPC, C 8 ) under optimum conditions (50 °C and pH 8.5). After two rounds… read more here.
Keywords: catalytic efficiency; esterase; activity; substrate affinity ... See more keywords
Loop engineering reveals the importance of active-site-decorating loops and gating residue in substrate affinity modulation of arginine deiminase (an anti-tumor enzyme).
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.03.134
Abstract: Protein engineering of enzyme loop regions is an effective strategy to improve enzymatic properties. Previous studies that aimed to boost the activity of PpADI (an arginine deiminase from Pseudomonas plecoglossicida) under physiological conditions yielded several… read more here.
Keywords: substrate affinity; active site; arginine deiminase; site ... See more keywords
Biochemical characterisation of fumarase C from a unicellular cyanobacterium demonstrating its substrate affinity, altered by an amino acid substitution
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-47025-7
Abstract: The tricarboxylic acid cycle produces NADH for oxidative phosphorylation and fumarase [EC 4.2.1.2] is a critical enzyme in this cycle, catalysing the reversible conversion of fumarate and l-malate. Fumarase is applied to industrial l-malate production… read more here.
Keywords: amino acid; malate; substrate affinity; substitution ... See more keywords
Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology for Biofuels"
DOI: 10.1186/s13068-019-1599-0
Abstract: BackgroundTo produce second-generation biofuels, enzymatic catalysis is required to convert cellulose from lignocellulosic biomass into fermentable sugars. β-Glucosidases finalize the process by hydrolyzing cellobiose into glucose, so the efficiency of cellulose hydrolysis largely depends on… read more here.
Keywords: bgl; production; substrate affinity; lignocellulosic biomass ... See more keywords
Investigation of changes in protein stability and substrate affinity of 3CL-protease of SARS-CoV-2 caused by mutations
Sign Up to like & getrecommendations! Published in 2022 at "Genetics and Molecular Biology"
DOI: 10.1590/1678-4685-gmb-2021-0404
Abstract: Abstract 3CLpro of SARS-CoV-2 is one of the enzymes required for the replication process of the virus responsible for the COVID-19 pandemic. In this study, changes in protein stability and substrate affinity caused by mutations… read more here.
Keywords: substrate affinity; protein stability; changes protein; stability ... See more keywords
Substrate Affinity Is Not Crucial for Therapeutic L-Asparaginases: Antileukemic Activity of Novel Bacterial Enzymes
Sign Up to like & getrecommendations! Published in 2024 at "Molecules"
DOI: 10.3390/molecules29102272
Abstract: L-asparaginases are used in the treatment of acute lymphoblastic leukemia. The aim of this work was to compare the antiproliferative potential and proapoptotic properties of novel L-asparaginases from different structural classes, viz. EcAIII and KpAIII… read more here.
Keywords: crucial therapeutic; ecaii; cell; affinity crucial ... See more keywords