Regulating substrate preference of phosphatase by reshaping the “cap domain” for multi‐enzymatic biosynthesis of high‐purity monosaccharides
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28244
Abstract: Phosphatases are a class of enzymes catalyzing the cleavage of monophosphate ester bonds from the phosphorylated substrates. They have important applications in construction of in vitro multi‐enzymatic system for monosaccharides. However, the enzymes generally show… read more here.
Keywords: biosynthesis; cap domain; multi enzymatic; high purity ... See more keywords
A novel d-2-hydroxy acid dehydrogenase with high substrate preference for phenylpyruvate originating from lactic acid bacteria: Structural analysis on the substrate specificity.
Sign Up to like & getrecommendations! Published in 2019 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2019.02.008
Abstract: 2-Hydroxy acid dehydrogenases (2-HADHs) have been implicated in the synthesis of 2-hydroxy acids from 2-oxo acids that are used in wide areas of industry. d-lactate dehydrogenases (d-LDHs), a subfamily of 2-HADH, have been utilized to… read more here.
Keywords: high substrate; substrate preference; acid; hydroxy acid ... See more keywords
The lipid substrate preference of CETP controls the biochemical properties of HDL in fat/cholesterol-fed hamsters
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Lipid Research"
DOI: 10.1016/j.jlr.2021.100027
Abstract: Abstract Cholesteryl ester transfer protein (CETP) modulates lipoprotein metabolism by transferring cholesteryl ester (CE) and triglyceride (TG) between lipoproteins. However, differences in the way CETP functions exist across species. Unlike human CETP, hamster CETP prefers… read more here.
Keywords: hamster cetp; cholesterol; human cetp; cetp ... See more keywords
Importance of Asparagine 202 in Manipulating Active Site Structure and Substrate Preference for Human CYP17A1.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00023
Abstract: The multifunctional cytochrome P450 17A1 (CYP17A1) plays a crucial role in human steroid hormone synthesis (UniProtKB─P05093). It first carries out standard monooxygenase chemistry, converting pregnenolone (PREG) and progesterone (PROG) into 17OH-PREG and 17OH-PROG, utilizing a… read more here.
Keywords: cyp17a1; asparagine; preg; active site ... See more keywords
Microbial substrate preference dictated by energy demand, not supply
Sign Up to like & getrecommendations! Published in 2017 at "Nature geoscience"
DOI: 10.1038/ngeo2978
Abstract: Growth substrates that maximize energy yield are widely thought to be utilized preferentially by microorganisms. However, observed distributions of microorganisms and their activities often deviate from predictions based solely on thermodynamic considerations of substrate energy… read more here.
Keywords: supply; energy demand; energy; substrate preference ... See more keywords
A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-03144-8
Abstract: Identification of novel enzymes from lignin degrading microorganisms will help to develop biotechnologies for biomass valorization and aromatic hydrocarbons degradation. Bacillus ligniniphilus L1 grows with alkaline lignin as the single carbon source and is a… read more here.
Keywords: ligniniphilus catechol; catechol; bacillus ligniniphilus; catechol dioxygenase ... See more keywords
Geometric Remodeling of Nitrilase Active Pocket Based on ALF-Scanning Strategy To Enhance Aromatic Nitrile Substrate Preference and Catalytic Efficiency
Sign Up to like & getrecommendations! Published in 2023 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00220-23
Abstract: In this study, the active pocket was largely remodeled based on the ALF-scanning strategy we developed. It is believed that ALF-scanning not only could be employed for substrate preference modification but might also play a… read more here.
Keywords: aromatic nitrile; alf scanning; active pocket; preference ... See more keywords
Mycobacterial OtsA Structures Unveil Substrate Preference Mechanism and Allosteric Regulation by 2-Oxoglutarate and 2-Phosphoglycerate
Sign Up to like & getrecommendations! Published in 2019 at "mBio"
DOI: 10.1128/mbio.02272-19
Abstract: Mycobacterial infections are a significant source of mortality worldwide, causing millions of deaths annually. Trehalose is a multipurpose disaccharide that plays a fundamental structural role in these organisms as a component of mycolic acids, a… read more here.
Keywords: mycobacterial otsa; oxoglutarate phosphoglycerate; otsa structures; substrate preference ... See more keywords
Endosomal v-ATPase as a Sensor Determining Myocardial Substrate Preference
Sign Up to like & getrecommendations! Published in 2022 at "Metabolites"
DOI: 10.3390/metabo12070579
Abstract: The heart is a metabolically flexible omnivore that can utilize a variety of substrates for energy provision. To fulfill cardiac energy requirements, the healthy adult heart mainly uses long-chain fatty acids and glucose in a… read more here.
Keywords: myocardial substrate; atpase; heart; substrate preference ... See more keywords