Substrate promiscuity of a de novo designed peroxidase.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of inorganic biochemistry"
DOI: 10.1016/j.jinorgbio.2021.111370
Abstract: The design and construction of de novo enzymes offer potentially facile routes to exploiting powerful chemistries in robust, expressible and customisable protein frameworks, while providing insight into natural enzyme function. To this end, we have… read more here.
Keywords: substrate promiscuity; peroxidase; promiscuity; promiscuity novo ... See more keywords
Molecular Basis for the Substrate Promiscuity of Isopentenyl Phosphate Kinase from Candidatus methanomethylophilus alvus.
Sign Up to like & getrecommendations! Published in 2021 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00655
Abstract: Isopentenyl phosphate kinases (IPKs) catalyze the ATP-dependent phosphorylation of isopentenyl monophosphate (IP) to isopentenyl diphosphate (IPP) in the alternate mevalonate pathways of the archaea and plant cytoplasm. In recent years, IPKs have also been employed… read more here.
Keywords: candidatus methanomethylophilus; substrate promiscuity; isopentenyl phosphate; non natural ... See more keywords
Elucidating Substrate Promiscuity within the FabI Enzyme Family.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00400
Abstract: The rapidly growing appreciation of enzymes' catalytic and substrate promiscuity may lead to their expanded use in the fields of chemical synthesis and industrial biotechnology. Here, we explore the substrate promiscuity of enoyl-acyl carrier protein… read more here.
Keywords: substrate promiscuity; within fabi; promiscuity; elucidating substrate ... See more keywords
Determinants and Prediction of Esterase Substrate Promiscuity Patterns.
Sign Up to like & getrecommendations! Published in 2018 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00996
Abstract: Esterases receive special attention because of their wide distribution in biological systems and environments and their importance for physiology and chemical synthesis. The prediction of esterases' substrate promiscuity level from sequence data and the molecular… read more here.
Keywords: substrate promiscuity; sequence data; promiscuity; prediction ... See more keywords
Engineering substrate promiscuity in 2,4-dichlorophenol hydroxylase by in silico design
Sign Up to like & getrecommendations! Published in 2018 at "RSC Advances"
DOI: 10.1039/c8ra03229g
Abstract: 2,4-Dichlorophenol hydroxylase (2,4-DCP hydroxylase) is a key enzyme in the degradation of 2,4-dichlorophenoxyacetic acid in the hydroxylation step in many bacteria. Our previous study demonstrated that a 2,4-DCP hydroxylase (TfdB-JLU) exhibits broad substrate specificity for… read more here.
Keywords: hydroxylase; substrate promiscuity; tfdb jlu;
Structural Insights into the Substrate Promiscuity of a Laboratory-Evolved Peroxygenase.
Sign Up to like & getrecommendations! Published in 2018 at "ACS Chemical Biology"
DOI: 10.2210/pdb6ekz/pdb
Abstract: Because of their minimal requirements, substrate promiscuity and product selectivity, fungal peroxygenases are now considered to be the jewel in the crown of C–H oxyfunctionalization biocatalysts. In this work, the crystal structure of the first… read more here.
Keywords: substrate promiscuity; evolved peroxygenase; structural insights; laboratory evolved ... See more keywords
Biological Filtering and Substrate Promiscuity Prediction for Annotating Untargeted Metabolomics
Sign Up to like & getrecommendations! Published in 2020 at "Metabolites"
DOI: 10.3390/metabo10040160
Abstract: Mass spectrometry coupled with chromatography separation techniques provides a powerful platform for untargeted metabolomics. Determining the chemical identities of detected compounds however remains a major challenge. Here, we present a novel computational workflow, termed extended… read more here.
Keywords: substrate promiscuity; biological filtering; model; metabolic model ... See more keywords