Protonation states of Asp residues in the human Nudix hydrolase MTH1 contribute to its broad substrate recognition.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS letters"
DOI: 10.1002/1873-3468.14611
Abstract: Human MutT homolog 1 (MTH1), also known as Nudix type motif 1 (NUDT1), hydrolyzes 8-oxo-dGTP and 2-oxo-dATP with broad substrate recognition and has attracted attention in anti-cancer therapeutics. Previous studies on MTH1 have proposed that… read more here.
Keywords: recognition; mth1; protonation; broad substrate ... See more keywords
Multisite Substrate Recognition in Asf1-Dependent Acetylation of Histone H3 K56 by Rtt109
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.07.005
Abstract: Rtt109 is a unique histone acetyltransferase acetylating histone H3 lysine 56 (H3K56), a modification critical for DNA replication-coupled nucleosome assembly and genome stability. In cells, histone chaperone Asf1 is essential for H3K56 acetylation, yet the… read more here.
Keywords: acetylation; substrate recognition; histone k56; rtt109 ... See more keywords
Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2018.11.005
Abstract: Isoprimeverose-producing enzymes (IPases) release isoprimeverose (α-d-xylopyranosyl-(1 → 6)-d-glucopyranose) from the non-reducing end of xyloglucan oligosaccharides. Aspergillus oryzae IPase (IpeA) is classified as a member of the glycoside hydrolase family 3 (GH3); however, it has unusual substrate specificity… read more here.
Keywords: ipea; crystal structure; isoprimeverose producing; substrate recognition ... See more keywords
Structure and substrate recognition by the Ruminococcus bromii amylosome pullulanases.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2021.107765
Abstract: Pullulanases are glycoside hydrolase family 13 (GH13) enzymes that target α1,6 glucosidic linkages within starch and aid in the degradation of the α1,4- and α1,6- linked glucans pullulan, glycogen and amylopectin. The human gut bacterium… read more here.
Keywords: substrate recognition; ruminococcus bromii; structure substrate; structure ... See more keywords
Substrate Recognition by the Peptidyl-(S)-2-mercaptoglycine Synthase TglHI during 3-Thiaglutamate Biosynthesis
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00087
Abstract: 3-Thiaglutamate is a recently identified amino acid analog originating from cysteine. During its biosynthesis, cysteinyl-tRNA is first enzymatically appended to the C-terminus of TglA, a 50-residue ribosomally translated peptide scaffold. After hydrolytic removal of the… read more here.
Keywords: biosynthesis; recognition peptidyl; tglhi; substrate recognition ... See more keywords
Substrate Recognition Mechanism of the Putative Yeast Carnosine N-methyltransferase.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00328
Abstract: Anserine (β-alanyl-N(Pi)-methyl-l-histidine) is a natural metabolite present in skeletal muscle and the central nervous system of vertebrates and plays important physiological roles in living organisms. The production of anserine is catalyzed by carnosine N-methyltransferases, which… read more here.
Keywords: carnosine; carnosine methyltransferase; substrate recognition;
Mapping the Substrate Recognition Pathway in Cytochrome P450.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b10840
Abstract: Cytochrome P450s are ubiquitous metalloenzymes involved in the metabolism and detoxification of foreign components via catalysis of the hydroxylation reactions of a vast array of organic substrates. However, the mechanism underlying the pharmaceutically critical process… read more here.
Keywords: cytochrome p450; catalytic center; substrate; substrate recognition ... See more keywords
PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
Sign Up to like & getrecommendations! Published in 2017 at "Cell Discovery"
DOI: 10.1038/celldisc.2017.27
Abstract: Protein phosphatase 2A (PP2A) is a major Ser/Thr phosphatase; it forms diverse heterotrimeric holoenzymes that counteract kinase actions. Using a peptidome that tiles the disordered regions of the human proteome, we identified proteins containing [LMFI]xx[ILV]xEx… read more here.
Keywords: phosphorylation; pp2a; substrate recognition; cytokinesis ... See more keywords
Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase
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DOI: 10.1038/nchembio.2494
Abstract: O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) is an essential human glycosyltransferase that adds O-GlcNAc modifications on numerous proteins. However, little is known about how OGT recognizes various protein substrates. Here we report GlcNAc electrophilic probes (GEPs)… read more here.
Keywords: recognition; electrophilic probes; substrate recognition; glcnac transferase ... See more keywords
Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-14794-z
Abstract: Core-fucosylation is an essential biological modification by which a fucose is transferred from GDP-β-L-fucose to the innermost N-acetylglucosamine residue of N -linked glycans. A single human enzyme α1,6-fucosyltransferase (FUT8) is the only enzyme responsible for… read more here.
Keywords: fut8; modification; catalysis; substrate recognition ... See more keywords
Substrate recognition diversity and transport dynamics of ABCC1
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-65501-9
Abstract: ABCC1 is an ATP-binding cassette (ABC) transporter that exports diverse endogenous and exogenous substrates, conferring resistance to many anticancer drugs and mediating various physiological functions. Here, we present ten cryo-EM structures of ABCC1 in different… read more here.
Keywords: recognition diversity; transport dynamics; substrate recognition; diversity transport ... See more keywords