Protonation states of Asp residues in the human Nudix hydrolase MTH1 contribute to its broad substrate recognition.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS letters"
DOI: 10.1002/1873-3468.14611
Abstract: Human MutT homolog 1 (MTH1), also known as Nudix type motif 1 (NUDT1), hydrolyzes 8-oxo-dGTP and 2-oxo-dATP with broad substrate recognition and has attracted attention in anti-cancer therapeutics. Previous studies on MTH1 have proposed that… read more here.
Keywords: recognition; mth1; protonation; broad substrate ... See more keywords
Multisite Substrate Recognition in Asf1-Dependent Acetylation of Histone H3 K56 by Rtt109
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.07.005
Abstract: Rtt109 is a unique histone acetyltransferase acetylating histone H3 lysine 56 (H3K56), a modification critical for DNA replication-coupled nucleosome assembly and genome stability. In cells, histone chaperone Asf1 is essential for H3K56 acetylation, yet the… read more here.
Keywords: acetylation; substrate recognition; histone k56; rtt109 ... See more keywords
Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2018.11.005
Abstract: Isoprimeverose-producing enzymes (IPases) release isoprimeverose (α-d-xylopyranosyl-(1 → 6)-d-glucopyranose) from the non-reducing end of xyloglucan oligosaccharides. Aspergillus oryzae IPase (IpeA) is classified as a member of the glycoside hydrolase family 3 (GH3); however, it has unusual substrate specificity… read more here.
Keywords: ipea; crystal structure; isoprimeverose producing; substrate recognition ... See more keywords
Structure and substrate recognition by the Ruminococcus bromii amylosome pullulanases.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2021.107765
Abstract: Pullulanases are glycoside hydrolase family 13 (GH13) enzymes that target α1,6 glucosidic linkages within starch and aid in the degradation of the α1,4- and α1,6- linked glucans pullulan, glycogen and amylopectin. The human gut bacterium… read more here.
Keywords: substrate recognition; ruminococcus bromii; structure substrate; structure ... See more keywords
Substrate Recognition by the Peptidyl-(S)-2-mercaptoglycine Synthase TglHI during 3-Thiaglutamate Biosynthesis
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00087
Abstract: 3-Thiaglutamate is a recently identified amino acid analog originating from cysteine. During its biosynthesis, cysteinyl-tRNA is first enzymatically appended to the C-terminus of TglA, a 50-residue ribosomally translated peptide scaffold. After hydrolytic removal of the… read more here.
Keywords: biosynthesis; recognition peptidyl; tglhi; substrate recognition ... See more keywords
Substrate Recognition Mechanism of the Putative Yeast Carnosine N-methyltransferase.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00328
Abstract: Anserine (β-alanyl-N(Pi)-methyl-l-histidine) is a natural metabolite present in skeletal muscle and the central nervous system of vertebrates and plays important physiological roles in living organisms. The production of anserine is catalyzed by carnosine N-methyltransferases, which… read more here.
Keywords: carnosine; carnosine methyltransferase; substrate recognition;
Mapping the Substrate Recognition Pathway in Cytochrome P450.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b10840
Abstract: Cytochrome P450s are ubiquitous metalloenzymes involved in the metabolism and detoxification of foreign components via catalysis of the hydroxylation reactions of a vast array of organic substrates. However, the mechanism underlying the pharmaceutically critical process… read more here.
Keywords: cytochrome p450; catalytic center; substrate; substrate recognition ... See more keywords
PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
Sign Up to like & getrecommendations! Published in 2017 at "Cell Discovery"
DOI: 10.1038/celldisc.2017.27
Abstract: Protein phosphatase 2A (PP2A) is a major Ser/Thr phosphatase; it forms diverse heterotrimeric holoenzymes that counteract kinase actions. Using a peptidome that tiles the disordered regions of the human proteome, we identified proteins containing [LMFI]xx[ILV]xEx… read more here.
Keywords: phosphorylation; pp2a; substrate recognition; cytokinesis ... See more keywords
Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase
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DOI: 10.1038/nchembio.2494
Abstract: O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) is an essential human glycosyltransferase that adds O-GlcNAc modifications on numerous proteins. However, little is known about how OGT recognizes various protein substrates. Here we report GlcNAc electrophilic probes (GEPs)… read more here.
Keywords: recognition; electrophilic probes; substrate recognition; glcnac transferase ... See more keywords
Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-14794-z
Abstract: Core-fucosylation is an essential biological modification by which a fucose is transferred from GDP-β-L-fucose to the innermost N-acetylglucosamine residue of N -linked glycans. A single human enzyme α1,6-fucosyltransferase (FUT8) is the only enzyme responsible for… read more here.
Keywords: fut8; modification; catalysis; substrate recognition ... See more keywords
Extensive substrate recognition by the streptococcal antibody-degrading enzymes IdeS and EndoS
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1101/2022.06.19.496714
Abstract: Enzymatic cleavage of IgG antibodies is a common strategy used by pathogenic bacteria to ablate immune effector function. The Streptococcus pyogenes bacterium secretes the protease IdeS and the glycosidase EndoS, which specifically catalyse cleavage and… read more here.
Keywords: recognition; extensive substrate; recognition streptococcal; substrate recognition ... See more keywords