BioWF: A Naturally‐Fused, Di‐Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200171
Abstract: The carbon backbone of biotin is constructed from the C7 di‐acid pimelate, which is converted to an acyl‐CoA thioester by an ATP‐dependent, pimeloyl‐CoA synthetase (PCAS, encoded by BioW). The acyl‐thioester is condensed with ʟ‐alanine in… read more here.
Keywords: fusion; biotin; domain; substrate ... See more keywords
Exploring the abundance of oleate hydratases in the genus Rhodococcus—discovery of novel enzymes with complementary substrate scope
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10627-7
Abstract: Oleate hydratases (Ohys, EC 4.2.1.53) are a class of enzymes capable of selective water addition reactions to a broad range of unsaturated fatty acids leading to the respective chiral alcohols. Much research was dedicated to… read more here.
Keywords: substrate scope; oleate hydratases; oleate; genus rhodococcus ... See more keywords
Expanding the Substrate Scope of a Bacterial Nucleotidyltransferase via Allosteric Mutations.
Sign Up to like & getrecommendations! Published in 2022 at "ACS infectious diseases"
DOI: 10.1021/acsinfecdis.2c00402
Abstract: Bacterial glycoconjugates, such as cell surface polysaccharides and glycoproteins, play important roles in cellular interactions and survival. Enzymes called nucleotidyltransferases use sugar-1-phosphates and nucleoside triphosphates (NTPs) to produce nucleoside diphosphate sugars (NDP-sugars), which serve as… read more here.
Keywords: nucleotidyltransferase; expanding substrate; ndp sugars; scope bacterial ... See more keywords
Investigating Substrate Scope and Enantioselectivity of a Defluorinase by a Stereochemical Probe.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b06019
Abstract: The possibility of a double Walden inversion mechanism of the fluoracetate dehalogenase FAcD (RPA1163) has been studied by subjecting rac-2-fluoro-2-phenyl acetic acid to the defluorination process. This stereochemical probe led to inversion of configuration in… read more here.
Keywords: substrate scope; stereochemical probe; scope enantioselectivity; investigating substrate ... See more keywords
Stannous chloride as a low toxicity and extremely cheap catalyst for regio-/site-selective acylation with unusually broad substrate scope
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DOI: 10.1039/d0gc02739a
Abstract: This work reports stannous chloride (SnCl2)-catalyzed regio-/site-selective acylation with unusually broad substrate scope. In addition to 1,2- and 1,3-diols and glycosides containing cis-vicinal diol, the substrate scope also includes glycosides without cis-vicinal diol. For such… read more here.
Keywords: site selective; regio site; selective acylation; substrate scope ... See more keywords
Substrate scope of a dehydrogenase from Sphingomonas species A1 and its potential application in the synthesis of rare sugars and sugar derivatives
Sign Up to like & getrecommendations! Published in 2018 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.13272
Abstract: Rare sugars and sugar derivatives that can be obtained from abundant sugars are of great interest to biochemical and pharmaceutical research. Here, we describe the substrate scope of a short‐chain dehydrogenase/reductase from Sphingomonas species A1… read more here.
Keywords: sugar derivatives; substrate scope; sugars sugar; rare sugars ... See more keywords