BioWF: A Naturally‐Fused, Di‐Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200171
Abstract: The carbon backbone of biotin is constructed from the C7 di‐acid pimelate, which is converted to an acyl‐CoA thioester by an ATP‐dependent, pimeloyl‐CoA synthetase (PCAS, encoded by BioW). The acyl‐thioester is condensed with ʟ‐alanine in… read more here.
Keywords: fusion; biotin; domain; substrate ... See more keywords
Engineering of a Baeyer‐Villiger monooxygenase to Improve Substrate Scope, Stereoselectivity and Regioselectivity
Sign Up to like & getrecommendations! Published in 2024 at "ChemBioChem"
DOI: 10.1002/cbic.202400328
Abstract: Baeyer‐Villiger monooxygenases belong to a family of flavin‐binding proteins that catalyze the Baeyer‐Villiger (BV) oxidation of ketones to produce lactones or esters, which are important intermediates in pharmaceuticals or sustainable materials. Phenylacetone monooxygenase (PAMO) from… read more here.
Keywords: baeyer villiger; monooxygenase; baeyer; substrate scope ... See more keywords
Ru‐Catalyzed Alkene Hydrophosphination: Correlating Substrate Scope with An Outer‐Sphere Mechanism
Sign Up to like & getrecommendations! Published in 2025 at "ChemCatChem"
DOI: 10.1002/cctc.202500153
Abstract: A detailed examination of substrate scope is described for alkene hydrophosphination catalyzed by a series of Cp*Ru complexes. An outer‐sphere mechanism is proposed that involves nucleophilic addition of a Ru phosphido ligand to the alkene… read more here.
Keywords: hydrophosphination; scope; substrate scope; sphere mechanism ... See more keywords
On the Substrate Scope of Dimethylsulfonium Propionate Lyases toward Dimethylsulfoxonium Propionate Derivatives
Sign Up to like & getrecommendations! Published in 2024 at "European Journal of Organic Chemistry"
DOI: 10.1002/ejoc.202400098
Abstract: The six dimethylsulfonium propionate (DMSP) lyases DddQ, DddW, DddP, DddY, DddK and DddL catalyze the elimination of dimethyl sulfide from DMSP and can also cleave the marine metabolite dimethylsulfoxonium propionate (DMSOP) to DMSO and acrylate.… read more here.
Keywords: dimethylsulfonium propionate; substrate scope; dimethylsulfoxonium propionate; propionate ... See more keywords
Exploring the abundance of oleate hydratases in the genus Rhodococcus—discovery of novel enzymes with complementary substrate scope
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10627-7
Abstract: Oleate hydratases (Ohys, EC 4.2.1.53) are a class of enzymes capable of selective water addition reactions to a broad range of unsaturated fatty acids leading to the respective chiral alcohols. Much research was dedicated to… read more here.
Keywords: substrate scope; oleate hydratases; oleate; genus rhodococcus ... See more keywords
Expanding the Substrate Scope of a Bacterial Nucleotidyltransferase via Allosteric Mutations.
Sign Up to like & getrecommendations! Published in 2022 at "ACS infectious diseases"
DOI: 10.1021/acsinfecdis.2c00402
Abstract: Bacterial glycoconjugates, such as cell surface polysaccharides and glycoproteins, play important roles in cellular interactions and survival. Enzymes called nucleotidyltransferases use sugar-1-phosphates and nucleoside triphosphates (NTPs) to produce nucleoside diphosphate sugars (NDP-sugars), which serve as… read more here.
Keywords: nucleotidyltransferase; expanding substrate; ndp sugars; scope bacterial ... See more keywords
Investigating Substrate Scope and Enantioselectivity of a Defluorinase by a Stereochemical Probe.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b06019
Abstract: The possibility of a double Walden inversion mechanism of the fluoracetate dehalogenase FAcD (RPA1163) has been studied by subjecting rac-2-fluoro-2-phenyl acetic acid to the defluorination process. This stereochemical probe led to inversion of configuration in… read more here.
Keywords: substrate scope; stereochemical probe; scope enantioselectivity; investigating substrate ... See more keywords
Broad substrate scope C-C oxidation in cyclodipeptides catalysed by a flavin-dependent filament
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-025-56127-y
Abstract: Cyclic dipeptides are produced by organisms in all domains of life. Many possess biological activities as anticancer and antimicrobial compounds. Oxidations are frequently present in these biologically active peptides. C-C bond oxidation is catalysed by… read more here.
Keywords: flavin dependent; oxidation cyclodipeptides; scope oxidation; substrate scope ... See more keywords
Substrate scope expansion of 4-phenol oxidases by rational enzyme selection and sequence-function relations
Sign Up to like & getrecommendations! Published in 2024 at "Communications Chemistry"
DOI: 10.1038/s42004-024-01207-1
Abstract: Enzymes play an important role in organic synthesis thanks to their high selectivity, however, such selectivity limits their substrate scope and hampers their wide application. Here, the authors report an approach to rationally select enzymes… read more here.
Keywords: sequence; phenol; sequence function; substrate scope ... See more keywords
Stannous chloride as a low toxicity and extremely cheap catalyst for regio-/site-selective acylation with unusually broad substrate scope
Sign Up to like & getrecommendations! Published in 2020 at "Green Chemistry"
DOI: 10.1039/d0gc02739a
Abstract: This work reports stannous chloride (SnCl2)-catalyzed regio-/site-selective acylation with unusually broad substrate scope. In addition to 1,2- and 1,3-diols and glycosides containing cis-vicinal diol, the substrate scope also includes glycosides without cis-vicinal diol. For such… read more here.
Keywords: site selective; regio site; selective acylation; substrate scope ... See more keywords
Substrate scope of a dehydrogenase from Sphingomonas species A1 and its potential application in the synthesis of rare sugars and sugar derivatives
Sign Up to like & getrecommendations! Published in 2018 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.13272
Abstract: Rare sugars and sugar derivatives that can be obtained from abundant sugars are of great interest to biochemical and pharmaceutical research. Here, we describe the substrate scope of a short‐chain dehydrogenase/reductase from Sphingomonas species A1… read more here.
Keywords: sugar derivatives; substrate scope; sugars sugar; rare sugars ... See more keywords