Repurposing a bacterial prolidase for organophosphorus hydrolysis: reshaped catalytic cavity switches substrate selectivity.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.27455
Abstract: Enzyme promiscuity is critical to the acquisition of evolutionary plasticity in cells and can be recruited for high-value chemical synthesis or xenobiotic degradation. The molecular determinants of substrate ambiguity are essential to this activity; however,… read more here.
Keywords: substrate selectivity; repurposing bacterial; hydrolysis; bacterial prolidase ... See more keywords
A variable loop involved in the substrate selectivity of pinoresinol/lariciresinol reductase from Camellia sinensis.
Sign Up to like & getrecommendations! Published in 2019 at "Phytochemistry"
DOI: 10.1016/j.phytochem.2019.02.003
Abstract: Pinoresinol/lariciresinol reductase (PLR), an NADPH-dependent reductase that catalyzes the sequential reduction of pinoresinol into secoisolariciresinol via Lariciresinol, can lead to the structural and stereochemical diversity of lignans. The relationship between substrate-selective reaction of PLR and… read more here.
Keywords: pinoresinol lariciresinol; sinensis; reductase; substrate selectivity ... See more keywords
Structural basis of substrate selectivity of viperin.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.9b00741
Abstract: Viperin is a radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication by converting cytidine triphosphate (CTP) into 3-deoxy-3,4-didehydro-CTP and by additional undefined mechanisms operating through its N- and C-terminal domains. Here, we describe crystal structures… read more here.
Keywords: basis substrate; structural basis; ctp utp; substrate selectivity ... See more keywords
A Membrane-Embedded Macromolecular Catalyst with Substrate Selectivity in Live Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c11168
Abstract: Substrate selectivity is one of the most attractive features of natural enzymes from their "bind-to-catalyze" working flow and is thus a goal for the development of synthetic enzyme mimics that mediate abiotic transformations. However, despite… read more here.
Keywords: substrate selectivity; selectivity; macromolecular catalyst; membrane ... See more keywords
Substrate-Selectivity in Catalytic Photooxygenation Processes Using a Quinine-BODIPY System
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DOI: 10.1055/s-0039-1690796
Abstract: Substrate selectivity by means of synthetic catalysts remains a challenging topic in chemistry. Here, a catalytic system combining an iodo-BODIPY photosensitizer and quinine was evaluated in the competitive photooxygenation of non-hydrogen and hydrogen-bond-donor substrates. The… read more here.
Keywords: selectivity catalytic; system; photooxygenation; substrate selectivity ... See more keywords
Identification of Molecular Determinants in iRhoms1 and 2 That Contribute to the Substrate Selectivity of Stimulated ADAM17
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232112796
Abstract: The metalloprotease ADAM17 is a key regulator of the TNFα, IL-6R and EGFR signaling pathways. The maturation and function of ADAM17 is controlled by the seven-membrane-spanning proteins iRhoms1 and 2. The functional properties of the… read more here.
Keywords: adam17; stimulated shedding; substrate selectivity; shedding adam17 ... See more keywords