Cushing's syndrome mutant PKAL205R exhibits altered substrate specificity
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12562
Abstract: The PKAL205R hotspot mutation has been implicated in Cushing's syndrome through hyperactive gain‐of‐function PKA signaling; however, its influence on substrate specificity has not been investigated. Here, we employ the Proteomic Peptide Library (ProPeL) approach to… read more here.
Keywords: mutant pkal205r; cushing syndrome; substrate specificity; syndrome mutant ... See more keywords
Cloning and bacterial expression systems for recombinant human heparanase production: Substrate specificity investigation by docking of a putative heparanase substrate
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1582
Abstract: Human heparanase (HPSE) is an enzyme that degrades the extracellular matrix. It is implicated in a multiplicity of physiological and pathological processes encouraging angiogenesis and tumor metastasis. The protein is a heterodimer composed of a… read more here.
Keywords: heparanase; substrate; substrate specificity; expression ... See more keywords
Substrate specificity of an actively assembling amyloid catalyst
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DOI: 10.1002/bip.23003
Abstract: In the presence of Zn2+, the catalytic, amyloid‐forming peptide Ac‐IHIHIQI‐NH2, was found to exhibit enhanced selectivity for hydrophobic p‐nitrophenyl ester substrates while in the process of self‐assembly. As opposed to the substrate p‐nitrophenyl acetate, which… read more here.
Keywords: catalyst; actively assembling; phe onp; specificity actively ... See more keywords
Rational design of thiolase substrate specificity for metabolic engineering applications
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DOI: 10.1002/bit.26737
Abstract: Metabolic engineering efforts require enzymes that are both highly active and specific toward the synthesis of a desired output product to be commercially feasible. The 3‐hydroxyacid (3HA) pathway, also known as the reverse β‐oxidation or… read more here.
Keywords: chain; metabolic engineering; thiolase; thiolase substrate ... See more keywords
Ancestral Haloalkane Dehalogenases Show Robustness and Unique Substrate Specificity
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DOI: 10.1002/cbic.201700197
Abstract: Ancestral sequence reconstruction (ASR) represents a powerful approach for empirical testing structure‐function relationships of diverse proteins. We employed ASR to predict sequences of five ancestral haloalkane dehalogenases (HLDs) from the HLD‐II subfamily. Genes encoding the… read more here.
Keywords: haloalkane dehalogenases; substrate specificity; ancestral haloalkane;
The Structural Determinants Accounting for the Broad Substrate Specificity of the Quorum Quenching Lactonase GcL
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DOI: 10.1002/cbic.201900024
Abstract: Quorum quenching lactonases are enzymes capable of hydrolyzing lactones, including N‐acyl homoserine lactones (AHLs). AHLs are molecules known as signals in bacterial communication dubbed quorum sensing. Bacterial signal disruption by lactonases was previously reported to… read more here.
Keywords: broad substrate; lactonase; quorum quenching; substrate specificity ... See more keywords
Multipurpose cellulases of Promicromonospora sp. VP111, with broad substrate specificity and tolerance properties.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of basic microbiology"
DOI: 10.1002/jobm.202200679
Abstract: Cellulolytic actinobacterium, Promicromonospora sp. VP111 concomitantly produced cellulases (CELs), xylanase and pectinase when grown on commercial cellulose and untreated agricultural lignocellulosic residues (wheat straw and sugarcane bagasse). Secreted CELs hydrolyzed (enhanced with Co2+ ion) multiple… read more here.
Keywords: substrate specificity; tolerance; promicromonospora vp111; broad substrate ... See more keywords
A comprehensive overview of substrate specificity of glycoside hydrolases and transporters in the small intestine
Sign Up to like & getrecommendations! Published in 2020 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-020-03564-1
Abstract: The human body is able to process and transport a complex variety of carbohydrates, unlocking their nutritional value as energy source or as important building block. The endogenous glycosyl hydrolases (glycosidases) and glycosyl transporter proteins… read more here.
Keywords: specificity glycoside; overview substrate; overview; small intestine ... See more keywords
Structural insight into the substrate specificity of PLP fold type IV transaminases
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DOI: 10.1007/s00253-020-10369-6
Abstract: Pyridoxal-5′-phosphate-dependent transaminases of fold type IV (class IV) are promising enzymes for ( R )-selective amination of organic compounds. Transaminases of fold type IV exhibit either strict ( R )-selectivity or ( S )-selectivity that… read more here.
Keywords: amino acid; structural insight; fold type; substrate specificity ... See more keywords
Substrate specificity among Diptera in decaying bioenergy wood: can they be conserved by the same measures as are currently applied to beetles?
Sign Up to like & getrecommendations! Published in 2020 at "Biodiversity and Conservation"
DOI: 10.1007/s10531-020-01992-w
Abstract: Although threatened by forestry, our knowledge concerning saproxylic insects is strongly biased towards well-known orders, mainly beetles (Coleoptera). The beetles have, therefore, formed the basis on which conservation measures of other groups have been formulated.… read more here.
Keywords: insects; specificity among; bioenergy wood; wood ... See more keywords
Determination of the Crystal Structure and Substrate Specificity of Ananain.
Sign Up to like & getrecommendations! Published in 2019 at "Biochimie"
DOI: 10.1016/j.biochi.2019.07.011
Abstract: Ananain (EC 3.4.22.31) accounts for less than 10% of the total enzyme in the crude pineapple stem extract known as bromelain, yet yields the majority of the proteolytic activity of bromelain. Despite a high degree… read more here.
Keywords: bromelain; ananain; specificity ananain; crystal ... See more keywords