A feature-guided, focused 3D signal permutation method for subtomogram averaging.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2022.107851
Abstract: Advances in electron microscope instrumentation, cryo-electron tomography data collection, and subtomogram averaging have allowed for the in-situ visualization of molecules and their complexes in their native environment. Current data processing pipelines commonly extract subtomograms as… read more here.
Keywords: feature guided; subtomogram averaging; signal permutation; permutation ... See more keywords
A Complete Workflow for Subnanometer Resolution Subtomogram Averaging In Situ
Sign Up to like & getrecommendations! Published in 2020 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927620018735
Abstract: Subtomogram averaging provides capabilities normal CryoEM single particle reconstruction does not. First, it permits study of highly flexible assemblies in which the single view provided by single particle analysis produces ambiguous results. More importantly, however,… read more here.
Keywords: resolution; subnanometer resolution; complete workflow; subtomogram averaging ... See more keywords
Strategies for picking membrane-associated particles within subtomogram averaging workflows
Sign Up to like & getrecommendations! Published in 2022 at "Faraday Discussions"
DOI: 10.1039/d2fd00022a
Abstract: Cryo-electron tomography (cryo-ET) with subtomogram averaging (STA) has emerged as a key tool for determining macromolecular structure(s) in vitro and in situ. However, processing cryo-ET data with STA currently requires significant user expertise. Recent efforts… read more here.
Keywords: membrane associated; subtomogram averaging; associated particles; strategies picking ... See more keywords
Current data processing strategies for cryo-electron tomography and subtomogram averaging
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical Journal"
DOI: 10.1042/bcj20200715
Abstract: Cryo-electron tomography (cryo-ET) can be used to reconstruct three-dimensional (3D) volumes, or tomograms, from a series of tilted two-dimensional images of biological objects in their near-native states in situ or in vitro. 3D subvolumes, or… read more here.
Keywords: processing strategies; electron tomography; cryo electron; subtomogram averaging ... See more keywords
A novel constrained reconstruction model towards high-resolution subtomogram averaging
Sign Up to like & getrecommendations! Published in 2021 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btz787
Abstract: MOTIVATION Electron tomography (ET) offers a unique capacity to image biological structures in situ. However, the resolution of ET reconstructed tomograms is not comparable to that of the single-particle cryo-EM. If many copies of the… read more here.
Keywords: reconstruction; resolution; subtomogram averaging; crm ... See more keywords