Reducing Agent‐Mediated Nonenzymatic Conversion of 2‐Oxoglutarate to Succinate: Implications for Oxygenase Assays
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.202000185
Abstract: l‐Ascorbate (l‐Asc) is often added to assays with isolated FeII‐ and 2‐oxoglutarate (2OG)‐dependent oxygenases to enhance activity. l‐Asc is proposed to be important in catalysis by some 2OG oxygenases in vivo. We report observations on the… read more here.
Keywords: nonenzymatic conversion; reducing agent; agent mediated; succinate ... See more keywords
Microbial interaction between the succinate‐utilizing bacterium Phascolarctobacterium faecium and the gut commensal Bacteroides thetaiotaomicron
Sign Up to like & getrecommendations! Published in 2020 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.1111
Abstract: A large variety of microbes are present in the human gut, some of which are considered to interact with each other. Most of these interactions involve bacterial metabolites. Phascolarctobacterium faecium hardly uses carbohydrates for growth… read more here.
Keywords: commensal bacteroides; succinate; faecium; phascolarctobacterium faecium ... See more keywords
Enhancement of succinate yield by manipulating NADH/NAD+ ratio and ATP generation
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8127-6
Abstract: We previously engineered Escherichia coli YL104 to efficiently produce succinate from glucose. In this study, we investigated the relationships between the NADH/NAD+ ratio, ATP level, and overall yield of succinate production by using glucose as… read more here.
Keywords: nad ratio; nadh nad; atp level; succinate ... See more keywords
Reduction of Fumarate to Succinate Mediated by Fusobacterium varium
Sign Up to like & getrecommendations! Published in 2018 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-018-2817-0
Abstract: Accumulation of succinate as a fermentation product of Fusobacterium varium was enhanced when the anaerobic bacterium was grown on complex peptone medium supplemented with fumarate. Residual substrates and fermentation products were determined by proton NMR… read more here.
Keywords: fumarate; succinate; fumarate succinate; fusobacterium varium ... See more keywords
Identification and characterization of Methyl succinate from Bacillus velezensis AR1
Sign Up to like & getrecommendations! Published in 2020 at "Australasian Plant Pathology"
DOI: 10.1007/s13313-020-00701-z
Abstract: Identification of antifungal organic compound from B. velezensis AR1 culture supernatant was performed using high-performance liquid chromatography (HPLC) and subjected to proton (1H) and carbon (13C) nuclear magnetic resonance (NMR). From the analysis of the… read more here.
Keywords: velezensis ar1; characterization methyl; succinate; identification ... See more keywords
Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate by increasing the intracellular FAD pool
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2019.03.015
Abstract: Abstract In this study, the FAD synthesis pathway was manipulated to increase its cellular concentration and thereby improve the electroactivity of E. coli. In a microbial electrosynthesis (MES) system with neutral red as electron carrier… read more here.
Keywords: microbial electrosynthesis; coli microbial; engineering electroactive; succinate ... See more keywords
Succinate Can Shuttle Reducing Power from the Hypoxic Retina to the O2-Rich Pigment Epithelium.
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.107606
Abstract: When O2 is plentiful, the mitochondrial electron transport chain uses it as a terminal electron acceptor. However, the mammalian retina thrives in a hypoxic niche in the eye. We find that mitochondria in retinas adapt… read more here.
Keywords: retina; succinate; succinate shuttle; pigment epithelium ... See more keywords
A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.109521
Abstract: The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology. However, the role that bacterial metabolites play in communicating with host cells during inflammatory diseases is poorly understood. Here, we aim… read more here.
Keywords: transepithelial pathway; succinate; pathway delivers; delivers succinate ... See more keywords
Kinetic studies of biocatalyzed copolyesters of poly(butylene succinate) (PBS) containing fully bio-based dilinoleic diol
Sign Up to like & getrecommendations! Published in 2019 at "European Polymer Journal"
DOI: 10.1016/j.eurpolymj.2019.04.038
Abstract: Abstract A series of segmented bio-copolyesters based on crystallizable hard segments of poly(butylene succinate) (PBS) and fully bio-based dilinoleic diol (DLAOH), creating dilinoleic succinate (DLS) soft segments, were prepared by enzymatically catalyzed polycondensation. The kinetic… read more here.
Keywords: succinate; poly butylene; bio based; fully bio ... See more keywords
One-step enzymatic synthesis of poly(p-dioxanone-co-butylene-co-succinate) copolyesters with well-defined structure and enhanced degradability
Sign Up to like & getrecommendations! Published in 2017 at "Polymer"
DOI: 10.1016/j.polymer.2017.01.055
Abstract: Abstract In this work, we present a mild one-step enzymatic-catalyzed copolymerization of diethyl succinate, 1,4-butanediol and p-dioxanone (PDO) to synthesize poly(p-dioxanone-co-butylene-co-succinate) copolyesters. Their chemical structure, triad distributions, and molecular weight of the copolyesters are characterized… read more here.
Keywords: butylene succinate; dioxanone; succinate; poly dioxanone ... See more keywords
Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation
Sign Up to like & getrecommendations! Published in 2018 at "Cell Stem Cell"
DOI: 10.1016/j.stem.2018.01.020
Abstract: Summary Neural stem cell (NSC) transplantation can influence immune responses and suppress inflammation in the CNS. Metabolites, such as succinate, modulate the phenotype and function of immune cells, but whether and how NSCs are also… read more here.
Keywords: extracellular succinate; succinate; macrophage derived; stem ... See more keywords