Energetics of the Glycosyl Transfer Reactions of Sucrose Phosphorylase.
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DOI: 10.1021/acs.biochem.3c00080
Abstract: From its structure and mechanism, sucrose phosphorylase is a specialized glycoside hydrolase that uses phosphate ions instead of water as the nucleophile of the reaction. Unlike the hydrolysis reaction, the phosphate reaction is readily reversible… read more here.
Keywords: transition state; energetics glycosyl; glycosyl transfer; reaction ... See more keywords
Reversibility of a Point Mutation Induced Domain Shift: Expanding the Conformational Space of a Sucrose Phosphorylase
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-28802-2
Abstract: Despite their popularity as enzyme engineering targets structural information about Sucrose Phosphorylases remains scarce. We recently clarified that the Q345F variant of Bifidobacterium adolescentis Sucrose Phosphorylase is able to accept large polyphenolic substrates like resveratrol… read more here.
Keywords: sucrose phosphorylase; reversibility point; domain shift;