Evaluation of suitable reference genes for normalization of quantitative reverse transcription PCR analyses in Clavibacter michiganensis
Sign Up to like & getrecommendations! Published in 2019 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.928
Abstract: Clavibacter michiganensis, the causal agent of bacterial canker of tomato, is a Gram‐positive bacterium and a model for studying plant diseases. The real‐time quantitative reverse transcription PCR (real‐time qRT‐PCR) assay is widely used to quantify… read more here.
Keywords: reference genes; suitable reference; michiganensis; gene expression ... See more keywords
Suitable reference tissues for quantitative susceptibility mapping of the brain
Sign Up to like & getrecommendations! Published in 2017 at "Magnetic Resonance in Medicine"
DOI: 10.1002/mrm.26369
Abstract: Since quantitative susceptibility mapping (QSM) quantifies magnetic susceptibility relative to a reference value, a suitable reference tissue has to be available to compare different subjects and stages of disease. read more here.
Keywords: susceptibility; suitable reference; reference tissues; reference ... See more keywords
Identification of suitable reference genes in leaves and roots of rapeseed (Brassica napus L.) under different nutrient deficiencies
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Integrative Agriculture"
DOI: 10.1016/s2095-3119(16)61436-3
Abstract: Abstract Nutrient deficiency stresses often occur simultaneously in soil. Thus, it's necessary to investigate the mechanisms underlying plant responses to multiple stresses through identification of some key stress-responsive genes. Quantitative real-time PCR (qRT-PCR) is essential… read more here.
Keywords: suitable reference; reference; nutrient deficiencies; different nutrient ... See more keywords
Establishment of suitable reference genes for studying relative gene expression during the transition from trophozoites to cyst-like stages and first evidences of stress-induced expression of meiotic genes in Trichomonas vaginalis.
Sign Up to like & getrecommendations! Published in 2021 at "Parasitology"
DOI: 10.1017/s0031182021000585
Abstract: Trichomonas vaginalis is a parasite of the human urogenital tract and the causative agent of trichomoniasis, a sexually transmitted disease of worldwide importance. This parasite is usually found as a motile flagellated trophozoite. However, when… read more here.
Keywords: expression; like stages; suitable reference; cyst like ... See more keywords
Selection of suitable reference genes for qRT-PCR normalisation under different experimental conditions in Eucommia ulmoides Oliv
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DOI: 10.1038/s41598-018-33342-w
Abstract: Normalisation of data, by choosing the appropriate reference genes, is fundamental for obtaining reliable results in quantitative real-time PCR (qPCR). This study evaluated the expression stability of 11 candidate reference genes with different varieties, developmental… read more here.
Keywords: selection suitable; normalisation; suitable reference; reference ... See more keywords
Selection and evaluation of suitable reference genes for quantitative gene expression analysis during infection of Cucumis sativus with Pectobacterium brasiliense.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of applied microbiology"
DOI: 10.1111/jam.15481
Abstract: AIMS Bacterial soft rot caused by Pectobacterium brasiliense (Pbr) has resulted in severe economic losses of cucumber production in northern China. Quantitative reverse transcription PCR (RT-qPCR) is widely used to determine the fold change in… read more here.
Keywords: reference genes; expression; reference; pectobacterium ... See more keywords
Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress
Sign Up to like & getrecommendations! Published in 2017 at "BMC Genomics"
DOI: 10.1186/s12864-017-3974-1
Abstract: BackgroundHigh ambient temperature is known to affect fish gonadal development and physiology in a variety of ways depending on the severity and duration of exposure; however, the underlying molecular mechanisms are poorly understood. Gonadal gene… read more here.
Keywords: analysis; suitable reference; gene expression; reference ... See more keywords