Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6625-7_19
Abstract: The fluorogenic probe dihydroethidium (DHE) is widely used for detecting intracellular superoxide. DHE oxidation by superoxide generates specifically the compound 2-hydroxyethidium (2-E+OH), so that 2-E+OH detection confers specificity to superoxide assessment among many other reactive… read more here.
Keywords: hplc; oxidation; dihydroethidium; superoxide ... See more keywords
Construction of a novel electrochemical sensing platform to investigate the effect of temperature on superoxide anions from cells and superoxide dismutase enzyme activity.
Sign Up to like & getrecommendations! Published in 2022 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2022.339561
Abstract: It is of great significance to realize the detection of trace amounts of superoxide anions (O2•-) in biological systems due to the connection between the concentration of O2•- and some diseases. Here, we present a… read more here.
Keywords: superoxide; superoxide anions; sensing platform; effect temperature ... See more keywords
Redox Dependent Activation of NF-kB Signaling in Cancer via Superoxide-Induced Phosphorylation of IKK
Sign Up to like & getrecommendations! Published in 2017 at "Free Radical Biology and Medicine"
DOI: 10.1016/j.freeradbiomed.2017.10.161
Abstract: Evidence over the years has highlighted the importance of intracellular redox status in determining cell fate. To date, our work has shown that a pro-oxidant intracellular milieu, particularly a mild increase in superoxide (O2•‒) promotes… read more here.
Keywords: superoxide; activation; cancer; redox dependent ... See more keywords
Superoxide induced inhibition of death receptor signaling is mediated via induced expression of apoptosis inhibitory protein cFLIP
Sign Up to like & getrecommendations! Published in 2019 at "Redox Biology"
DOI: 10.1016/j.redox.2019.101403
Abstract: The death inhibitory proteins, cFLIP and Bcl-2, canonically act at different steps to regulate receptor-mediated apoptosis in cancer cells. Here we report that pharmacological or genetic means to effect an increase in intracellular superoxide result… read more here.
Keywords: death; apoptosis; superoxide; cflip ... See more keywords
Potassium Superoxide: A Unique Alternative for Metal-Air Batteries.
Sign Up to like & getrecommendations! Published in 2018 at "Accounts of chemical research"
DOI: 10.1021/acs.accounts.8b00332
Abstract: Lithium-oxygen (Li-O2) batteries have been envisaged and pursued as the long-term successor to Li-ion batteries, due to the highest theoretical energy density among all known battery chemistries. However, their practical application is hindered by low… read more here.
Keywords: oxygen; chemistry; superoxide; metal ... See more keywords
Self-Damaging Aerobic Reduction of Graphene Oxide by Escherichia coli: Role of GO-Mediated Extracellular Superoxide Formation.
Sign Up to like & getrecommendations! Published in 2018 at "Environmental science & technology"
DOI: 10.1021/acs.est.8b03753
Abstract: Microbial reduction of graphene oxide (GO) under aerobic conditions is poorly understood despite its critical role in changing GO toxicity and environmental fate. Here we show that 20 mg/L GO interacts with the membrane-bound cytochrome… read more here.
Keywords: graphene oxide; reduction; reduction graphene; superoxide ... See more keywords
Functionalized Quartz Nanopipette for Intracellular Superoxide Sensing: A Tool for Monitoring Reactive Oxygen Species Levels in Single Living Cell.
Sign Up to like & getrecommendations! Published in 2018 at "ACS sensors"
DOI: 10.1021/acssensors.8b00185
Abstract: Reactive oxygen species (ROS), including superoxide radical anions, are vital components in numerous biological functions, including cell signaling and immune responses. Since ROS react with other biomolecules and oxidize them quickly, it is essential for… read more here.
Keywords: reactive oxygen; oxygen species; single living; superoxide ... See more keywords
Role of Superoxide Reductase FA796 in Oxidative Stress Resistance in Filifactor alocis
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-65806-3
Abstract: Filifactor alocis, a Gram-positive anaerobic bacterium, is now a proposed diagnostic indicator of periodontal disease. Because the stress response of this bacterium to the oxidative environment of the periodontal pocket may impact its pathogenicity, an… read more here.
Keywords: fa796; superoxide; stress resistance; stress ... See more keywords
Reaction of a nitrosyl complex of Mn(II)-porphyrinate with superoxide: NOD activity is favoured over SOD activity.
Sign Up to like & getrecommendations! Published in 2023 at "Dalton transactions"
DOI: 10.1039/d3dt00570d
Abstract: A five-coordinated {Mn(NO)}6 complex of Mn(II)-porphyrinate, [Mn(TMPP2-)(NO)], 1 {TMPPH2 = 5,10,15,20-tetrakis(4-methoxyphenyl)porphyrin}, upon reaction with two equivalents of superoxide (O2-) in THF at -40 °C results in the corresponding MnIII-OH complex [MnIII(TMPP2-)(OH)], 2, via the formation… read more here.
Keywords: superoxide; complex porphyrinate; equivalent superoxide; reaction ... See more keywords
Siderophores provoke extracellular superoxide production by Arthrobacter strains during carbon sources-level fluctuation.
Sign Up to like & getrecommendations! Published in 2022 at "Environmental microbiology"
DOI: 10.1111/1462-2920.15914
Abstract: Superoxide and other reactive oxygen species (ROS) shape microbial communities and drive the transformation of metals and inorganic/organic matter. Taxonomically diverse bacteria and phytoplankton produce extracellular superoxide during laboratory cultivation. Understanding the physiological reasons for… read more here.
Keywords: superoxide; superoxide production; sources level; carbon sources ... See more keywords
Stimulation of RAS-dependent ROS signaling extends longevity by modulating a developmental program of global gene expression
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.adc9851
Abstract: We show that elevation of mitochondrial superoxide generation increases Caenorhabditis elegans life span by enhancing a RAS-dependent ROS (reactive oxygen species) signaling pathway (RDRS) that controls the expression of half of the genome as well… read more here.
Keywords: superoxide; ras dependent; dependent ros; program ... See more keywords