Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Journal"
DOI: 10.1002/biot.202000231
Abstract: Generation of high‐affinity monoclonal antibodies by immunization of chickens is a valuable strategy, particularly for obtaining antibodies directed against epitopes that are conserved in mammals. A generic procedure is established for the humanization of chicken‐derived… read more here.
Keywords: affinity; yeast surface; chicken; humanization ... See more keywords
Amber suppression coupled with inducible surface display identifies cells with high recombinant protein productivity
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26892
Abstract: Cell line development (CLD) for biotherapeutics is a time‐ and resource‐intensive process requiring the isolation and screening of large numbers of clones to identify high producers. Novel methods aimed at enhancing cell line screening efficiency… read more here.
Keywords: productivity; cell; membrane; surface display ... See more keywords
Directed Evolution of Protein Thermal Stability Using Yeast Surface Display.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6857-2_4
Abstract: Yeast surface display is a powerful protein engineering technology that has been used for many applications including engineering protein stability. Direct screening for improved thermal stability can be accomplished by heat shock of yeast displayed… read more here.
Keywords: protein; engineering; yeast surface; stability ... See more keywords
Isolation of Antigen-Specific VHH Single-Domain Antibodies by Combining Animal Immunization with Yeast Surface Display.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_10
Abstract: In addition to conventional hetero-tetrameric antibodies, the adaptive immune repertoire of camelids comprises the so-called heavy chain-only antibodies devoid of light chains. Consequently, antigen binding is mediated solely by the variable domain of the heavy… read more here.
Keywords: isolation antigen; yeast surface; antigen specific; single domain ... See more keywords
Rapid Generation of Chicken Immune Libraries for Yeast Surface Display.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_16
Abstract: Fluorescence-activated cell sorting (FACS) in combination with yeast surface display has emerged as a vital tool for the isolation and engineering of antibodies and antibody-derived fragments from synthetic, naïve, and immune libraries. However, the generation… read more here.
Keywords: generation; surface display; immune libraries; yeast surface ... See more keywords
New Bacterial Surface Display System Development and Application Based on Bacillus subtilis YuaB Biofilm Component as an Anchoring Motif
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-020-0397-7
Abstract: Bacterial surface display system has been adopted in various biotechnological applications. In the case of Bacillus subtilis , most of the studies have been developed using spore based surface display system utilizing the inherent rigidity… read more here.
Keywords: system; surface; display system; bacterial surface ... See more keywords
Expression and presentation of immune-related membrane proteins of fish by a cell surface display platform using insect cells.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular immunology"
DOI: 10.1016/j.molimm.2019.07.020
Abstract: Cell surface display is a useful platform to examine the interactions between two proteins of interest, such as immune receptors and ligands. This technique is also useful for studies on the immune receptors of lower… read more here.
Keywords: immune; surface; cell surface; cell ... See more keywords
Simultaneous surface display and cargo loading of encapsulin nanocompartments and their use for rational vaccine design.
Sign Up to like & getrecommendations! Published in 2018 at "Vaccine"
DOI: 10.1016/j.vaccine.2018.05.034
Abstract: In the past decades protein nanoparticles have successfully been used for vaccine applications. Their particulate nature and dense repetitive subunit organization makes them perfect carriers for antigen surface display and confers high immunogenicity. Nanoparticles have… read more here.
Keywords: protein; surface; encapsulin; vaccine ... See more keywords
Emerging enzyme surface display systems for waste resource recovery
Sign Up to like & getrecommendations! Published in 2022 at "Environmental Microbiology"
DOI: 10.1111/1462-2920.16284
Abstract: Abstract The current century marks an inflection point for human progress, as the developed world increasingly comes to recognize that the ecological and socioeconomic impacts of resource extraction must be balanced with more sustainable modes… read more here.
Keywords: resource recovery; display; display systems; surface display ... See more keywords
Antigen-43-mediated surface display revealed in Escherichia coli by different fusion sites and proteins
Sign Up to like & getrecommendations! Published in 2019 at "Bioresources and Bioprocessing"
DOI: 10.1186/s40643-019-0248-6
Abstract: BackgroundCell surface display system allows for endowing functional proteins expressed on bacterial surface by fusing different anchor proteins. Among PgsA, Blc, and Omp anchor, the antigen 43 (Ag43)-mediated surface display is a novel system in… read more here.
Keywords: surface; different fusion; surface display; fusion sites ... See more keywords
A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2020.614498
Abstract: Lactic acid bacteria (LAB) are a group of gut commensals increasingly recognized for their potential to deliver bioactive molecules in vivo. The delivery of therapeutic proteins, in particular, can be achieved by anchoring them to… read more here.
Keywords: cad4a; surface; cell anchoring; cell ... See more keywords