Structure‐based discovery of small molecules improving stability of human broadly‐neutralizing anti‐HIV antibody 2F5 in plant suspension cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100266
Abstract: The production of biopharmaceuticals in engineered plant‐based systems is a promising technology that has proven its suitability for the production of various recombinant glyco–proteins that are currently undergoing clinical trials. However, compared to mammalian cell… read more here.
Keywords: broadly neutralizing; human broadly; neutralizing anti; anti hiv ... See more keywords
Direct Reprogramming of Human Suspension Cells into Mesodermal Cell Lineages via Combined Magnetic Targeting and Photothermal Stimulation by Magnetic Graphene Oxide Complexes.
Sign Up to like & getrecommendations! Published in 2017 at "Small"
DOI: 10.1002/smll.201700703
Abstract: Suspension cells can provide a source of cells for cellular reprogramming, but they are difficult to transfect by nonviral vectors. An efficient and safe nonviral vector (GO-Fe3 O4 -PEI complexes) based on iron oxide nanoparticle… read more here.
Keywords: graphene oxide; magnetic targeting; photothermal stimulation; suspension cells ... See more keywords
A method for high transfection efficiency in THP-1 suspension cells without PMA treatment.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.12.032
Abstract: Adherent cells such as mouse RAW cells or human cancer U2OS cells are beneficial to DNA transfection, with 20%-60% transfection efficiency. However, this DNA transfection is rarely used on suspension cells due to its low… read more here.
Keywords: transfection efficiency; suspension cells; transfection; method ... See more keywords
SpinTip: A Simple, Robust, and Versatile Preanalytical Method for Microscale Suspension Cell Proteomics.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00478
Abstract: Sample loss and contamination are critical preanalytical pitfalls in microscale proteomic applications of nonadhering cells. Common assays and workflows are not easily adoptable to microscale sample sizes of suspension cells due to inadvertent sample loss.… read more here.
Keywords: cell; simple robust; suspension cell; suspension cells ... See more keywords
Production of Schisandrin A and Schisandrin B from Callus and Suspension Cell Cultures of Schisandra chinensis
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DOI: 10.1177/1934578x1701200626
Abstract: For establishing the fermentation system of synthetic schizandrin A and schizandrin B, culture conditions of the Schisandra chinensis callus and the suspension cell were studied in this paper. The friable calluses of Schisandra chinensis (Turcz.)… read more here.
Keywords: callus suspension; suspension cells; suspension; schisandrin ... See more keywords
Transcriptome Analysis Reveals Differentially Expressed Genes That Regulate Biosynthesis of the Active Compounds with Methyl Jasmonate in Rosemary Suspension Cells
Sign Up to like & getrecommendations! Published in 2021 at "Genes"
DOI: 10.3390/genes13010067
Abstract: To study the effects of Methyl jasmonates (MeJA) on rosemary suspension cells, the antioxidant enzymes’ change of activities under different concentrations of MeJA, including 0 (CK), 10 (M10), 50 (M50) and 100 μM MeJA (M100).… read more here.
Keywords: expressed genes; differentially expressed; suspension cells; rosemary suspension ... See more keywords
Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22031409
Abstract: The rice cell suspension culture system is a good way to produce recombinant human proteins, owing to its high biosafety and low production cost. Human Octamer-binding Transcription Factor 4 (Oct4) is a fundamental transcription factor… read more here.
Keywords: recombinant human; rice; suspension cells; suspension ... See more keywords