Identification of a new Z‐DNA inducer using SYBR green 1 as a DNA conformation sensor
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13513
Abstract: Z‐DNA, which is left‐handed double‐stranded DNA, is involved in various cellular processes. However, its biological roles have not been fully evaluated due to the lack of tools available that can control the precise conformational change… read more here.
Keywords: identification new; dna; new dna; dna inducer ... See more keywords
Fluorescence quenching of the SYBR Green I-dsDNA complex by in situ generated magnetic ionic liquids
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02538-3
Abstract: Magnetic ionic liquids (MILs) with metal-containing cations are promising extraction solvents that provide fast and high efficiency extraction of DNA. Hydrophobic MILs can be generated in situ in a methodology called in situ dispersive liquid-liquid… read more here.
Keywords: fluorescence quenching; fluorescence; green dsdna; sybr green ... See more keywords
Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
Sign Up to like & getrecommendations! Published in 2022 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-022-11822-4
Abstract: Abstract The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the… read more here.
Keywords: sybr green; primer sets; sars cov; based qpcr ... See more keywords
RPA-SYBR Green I based instrument-free visual detection for pathogenic Yersinia enterocolitica in meat.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114157
Abstract: Pathogenic Yersinia (Y.) enterocolitica is the primary causative agent of Yersiniosis, with outbreaks in numerous countries around the world, and causes diarrhea and vomiting in animals and humans. Therefore, an instrument-free and convenient nucleic acid… read more here.
Keywords: enterocolitica; sybr green; detection; rpa sybr ... See more keywords
Detection of phospholipase A2 in serum based on LRET mechanism between upconversion nanoparticles and SYBR green I.
Sign Up to like & getrecommendations! Published in 2021 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.11.025
Abstract: Phospholipase A2 (PLA2) may be a vital biomarker for the prediction and diagnosis of some diseases. Consequently, it is of great significance to quantitatively detect PLA2 in biologic samples. Herein, on the basis of the… read more here.
Keywords: upconversion nanoparticles; detection; pla2; phospholipase ... See more keywords
Aptamer truncation strategy assisted by molecular docking and sensitive detection of T-2 toxin using SYBR Green I as a signal amplifier.
Sign Up to like & getrecommendations! Published in 2022 at "Food chemistry"
DOI: 10.1016/j.foodchem.2022.132171
Abstract: Herein, for the first time, a novel truncation strategy for aptamers' sequences based on molecular docking was reported for the first time. T-2 toxin and AFB1 aptamers were employed as models and fluorescence polarization was… read more here.
Keywords: sybr green; truncation strategy; detection toxin; toxin ... See more keywords
SYBR Green real-time qPCR method: Diagnose drowning more rapidly and accurately.
Sign Up to like & getrecommendations! Published in 2021 at "Forensic science international"
DOI: 10.1016/j.forsciint.2021.110720
Abstract: In the field of drowning research, the method of diatom morphology has been most applied to determine whether the cause of death is drowning. However, the characteristics of complex operation, high level of professional knowledge… read more here.
Keywords: qpcr method; real time; method; sybr green ... See more keywords
Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.11.012
Abstract: TaqMan and SYBR Green quantitative PCR (qPCR) methods were developed as DNA-based approaches to reproducibly enumerate M13 and T7 phages from phage display selection experiments individually and simultaneously. The genome copies of M13 and T7… read more here.
Keywords: qpcr; phage; sybr green; green qpcr ... See more keywords
Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB
Sign Up to like & getrecommendations! Published in 2019 at "Molecular and Cellular Probes"
DOI: 10.1016/j.mcp.2018.12.004
Abstract: Abstract Acute hepatopancreatic necrosis disease (AHPND), also known as Early mortality syndrome (EMS), is a recently emerged lethal disease that has caused major economic losses in shrimp aquaculture. The etiologic agents are Vibrio spp. that… read more here.
Keywords: pira pirb; detection; real time; toxin genes ... See more keywords
Simultaneous detection of classical swine fever virus and porcine circovirus 3 by SYBR green I-based duplex real-time fluorescence quantitative PCR.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2020.101524
Abstract: In the present study, the SYBR green I-based duplex quantitative polymerase chain reaction (qPCR) was developed for simultaneous detection of classical swine fever virus (CSFV) and porcine circovirus 3 (PCV3). The assay was used to… read more here.
Keywords: detection; csfv; porcine; sybr green ... See more keywords
A novel quantitative PCR assay for testing bacteria directly from plate cultures using SYBR Green technology.
Sign Up to like & getrecommendations! Published in 2017 at "Pathology"
DOI: 10.1016/j.pathol.2017.06.004
Abstract: CI, confidence interval; CV, coefficient of variation; RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation. a Number of batches of 30 tests analysed for mecA gene using standard suspensions of methicillin resistant Staphylococcus aureus… read more here.
Keywords: detection; plate cultures; pcr; sybr green ... See more keywords