Real-time three-dimensional tracking of single synaptic vesicles reveals that synaptic vesicles undergoing kiss-and-run fusion remain close to their original fusion site before reuse.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.05.043
Abstract: The release of neurotransmitters via the fusion between synaptic vesicles and the presynaptic membrane is an essential step in synaptic transmission. Synaptic vesicles generally undergo two distinct modes of exocytosis called full-collapse fusion and kiss-and-run… read more here.
Keywords: three dimensional; synaptic vesicles; run fusion; fusion ... See more keywords
Membrane Defects Recruit C-Terminus of Complexin to Synaptic Vesicles
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.2138
Abstract: Single vesicle-vesicle fusion assays based on FRET of lipid or content mixing [1-3] are widely used to investigate complexin on vesicle docking [4] and fast fusion process [5]. Most recently, we discovered the important role… read more here.
Keywords: synaptic vesicles; diao; complexin; membrane defects ... See more keywords
Synaptotagmin-7 controls the size of the reserve and resting pools of synaptic vesicles in hippocampal neurons.
Sign Up to like & getrecommendations! Published in 2018 at "Cell calcium"
DOI: 10.1016/j.ceca.2018.06.004
Abstract: Continuous neurotransmitter release is subjected to synaptic vesicle availability, which in turn depends on vesicle recycling and the traffic of vesicles between pools. We studied the role of Synaptotagmin-7 (Syt-7) in synaptic vesicle accessibility for… read more here.
Keywords: reserve resting; hippocampal neurons; size reserve; vesicle ... See more keywords
Transient docking of synaptic vesicles: Implications and mechanisms
Sign Up to like & getrecommendations! Published in 2022 at "Current Opinion in Neurobiology"
DOI: 10.1016/j.conb.2022.102535
Abstract: As synaptic vesicles fuse, they must continually be replaced with new docked, fusion-competent vesicles to sustain neurotransmission. It has long been appreciated that vesicles are recruited to docking sites in an activity-dependent manner. However, once… read more here.
Keywords: vesicles implications; microscopy; implications mechanisms; transient docking ... See more keywords
Co-packaging of opposing neurotransmitters in individual synaptic vesicles in the central nervous system
Sign Up to like & getrecommendations! Published in 2022 at "Neuron"
DOI: 10.1016/j.neuron.2022.01.007
Abstract: Many mammalian neurons release multiple neurotransmitters to activate diverse classes of postsynaptic ionotropic receptors. Entopeduncular nucleus somatostatin (EP Sst+) projection neurons to the lateral habenula (LHb) release both glutamate and GABA, but it is unclear… read more here.
Keywords: opposing neurotransmitters; synaptic vesicles; packaging opposing; neurotransmitters individual ... See more keywords
Detection of Glutamate Encapsulated in Liposomes by Optical Trapping Raman Spectroscopy
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c07206
Abstract: The transmission of neuronal information is propagated through synapses by neurotransmitters released from presynapses to postsynapses. Neurotransmitters released from the presynaptic vesicles activate receptors on the postsynaptic membrane. Glutamate acts as a major excitatory neurotransmitter… read more here.
Keywords: optical trapping; synaptic vesicles; spectroscopy; glutamate ... See more keywords
Hidden proteome of synaptic vesicles in the mammalian brain
Sign Up to like & getrecommendations! Published in 2020 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2011870117
Abstract: Significance Mammalian central synapses of diverse functions contribute to highly complex brain organization, but the molecular basis of synaptic diversity remains open. This is because current synapse proteomics are restricted to the “average” composition of… read more here.
Keywords: proteome synaptic; hidden proteome; synaptic vesicles; brain ... See more keywords
Nanoscale architecture of synaptic vesicles and scaffolding complexes revealed by cryo-electron tomography
Sign Up to like & getrecommendations! Published in 2024 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2403136121
Abstract: Significance Imaging the ultrastructure and molecular architecture of synapses is essential to understanding synaptic neurotransmission. Scaffolding proteins on both sides of the synapse form subsynaptic clusters that are aligned across the synapse. This is thought… read more here.
Keywords: architecture synaptic; synaptic vesicles; scaffolding complexes; tomography ... See more keywords
Fast and slow recycling of synaptic vesicles
Sign Up to like & getrecommendations! Published in 2020 at "Science"
DOI: 10.1126/science.367.6477.523-e
Abstract: Neuroscience The function of microtubules and filamentous actin in mature synapses is not fully understood. Using confocal and stimulated emission depletion microscopy on a giant auditory synapse of the rat nervous system, termed the calyx… read more here.
Keywords: fast slow; microscopy; synaptic vesicles; filamentous actin ... See more keywords
Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin
Sign Up to like & getrecommendations! Published in 2025 at "BMC Biology"
DOI: 10.1186/s12915-025-02464-9
Abstract: The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule ends. Nonetheless,… read more here.
Keywords: brain; synaptic vesicles; tubulin; synaptic vesicle ... See more keywords
Anterograde transport of synaptic vesicles is modulated by vesicular reversals and stationary cargo clusters.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cell science"
DOI: 10.1242/jcs.261223
Abstract: Stationary clusters of vesicles are a prominent feature of axonal transport, but little is known about their physiological and functional relevance to axonal transport. We investigate the role of vesicle motility characteristics in modulating the… read more here.
Keywords: cargo; anterograde transport; synaptic vesicles; stationary clusters ... See more keywords