Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+
Sign Up to like & getrecommendations! Published in 2018 at "FEBS Letters"
DOI: 10.1002/1873-3468.13040
Abstract: Synaptotagmin‐1 (Syt1) is the primary calcium sensor (Ca2+) that mediates neurotransmitter release at the synapse. The tandem C2 domains (C2A and C2B) of Syt1 exhibit functionally critical, Ca2+‐dependent interactions with the plasma membrane. With the… read more here.
Keywords: synaptotagmin; rearrangements confinement; binding energy; ca2 ... See more keywords
Structural Impact of Phosphorylation and Dielectric Constant Variation on Synaptotagmin's IDR.
Sign Up to like & getrecommendations! Published in 2018 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.12.013
Abstract: We used time-resolved Förster resonance energy transfer, circular dichroism, and molecular dynamics simulation to investigate the structural dependence of synaptotagmin 1's intrinsically disordered region (IDR) on phosphorylation and dielectric constant. We found that a peptide… read more here.
Keywords: synaptotagmin; dielectric constant; idr; phosphorylation dielectric ... See more keywords
Membrane Association and Functional Mechanism of Synaptotagmin-1 in Triggering Vesicle Fusion.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.08.008
Abstract: Upon Ca2+ influx, synaptic vesicles fuse with the presynaptic plasma membrane (PM) to release neurotransmitters. Membrane fusion is triggered by synaptotagmin-1, a transmembrane protein in the vesicle membrane (VM), but the mechanism is under debate.… read more here.
Keywords: synaptotagmin; mechanism; vesicle; membrane association ... See more keywords
Synaptotagmin: Mechanisms of an electrostatic switch
Sign Up to like & getrecommendations! Published in 2020 at "Neuroscience Letters"
DOI: 10.1016/j.neulet.2020.134834
Abstract: Synaptic transmission relies on the fast, synchronous fusion of neurotransmitter filled vesicles with the presynaptic membrane. Synaptotagmin is the Ca2+ sensor that couples the Ca2+ influx into nerve terminals following an action potential with this… read more here.
Keywords: mechanisms electrostatic; synaptotagmin mechanisms; synaptotagmin; electrostatic switch ... See more keywords
Drosophila studies support a role for a presynaptic synaptotagmin mutation in a human congenital myasthenic syndrome
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0184817
Abstract: During chemical transmission, the function of synaptic proteins must be coordinated to efficiently release neurotransmitter. Synaptotagmin 2, the Ca2+ sensor for fast, synchronized neurotransmitter release at the human neuromuscular junction, has recently been implicated in… read more here.
Keywords: drosophila; synaptotagmin; congenital myasthenic; myasthenic syndrome ... See more keywords