Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+
Sign Up to like & getrecommendations! Published in 2018 at "FEBS Letters"
DOI: 10.1002/1873-3468.13040
Abstract: Synaptotagmin‐1 (Syt1) is the primary calcium sensor (Ca2+) that mediates neurotransmitter release at the synapse. The tandem C2 domains (C2A and C2B) of Syt1 exhibit functionally critical, Ca2+‐dependent interactions with the plasma membrane. With the… read more here.
Keywords: synaptotagmin; rearrangements confinement; binding energy; ca2 ... See more keywords
Evaluation of synaptotagmin‐1 action models by all‐atom molecular dynamics simulations
Sign Up to like & getrecommendations! Published in 2025 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13966
Abstract: Neurotransmitter release is triggered in microseconds by the two C2 domains of the Ca2+ sensor synaptotagmin‐1 and by SNARE complexes, which form four‐helix bundles that bridge the vesicle and plasma membranes. The synaptotagmin‐1 C2B domain… read more here.
Keywords: fusion; dynamics simulations; molecular dynamics; synaptotagmin ... See more keywords
Structural Impact of Phosphorylation and Dielectric Constant Variation on Synaptotagmin's IDR.
Sign Up to like & getrecommendations! Published in 2018 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.12.013
Abstract: We used time-resolved Förster resonance energy transfer, circular dichroism, and molecular dynamics simulation to investigate the structural dependence of synaptotagmin 1's intrinsically disordered region (IDR) on phosphorylation and dielectric constant. We found that a peptide… read more here.
Keywords: synaptotagmin; dielectric constant; idr; phosphorylation dielectric ... See more keywords
Membrane Association and Functional Mechanism of Synaptotagmin-1 in Triggering Vesicle Fusion.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.08.008
Abstract: Upon Ca2+ influx, synaptic vesicles fuse with the presynaptic plasma membrane (PM) to release neurotransmitters. Membrane fusion is triggered by synaptotagmin-1, a transmembrane protein in the vesicle membrane (VM), but the mechanism is under debate.… read more here.
Keywords: synaptotagmin; mechanism; vesicle; membrane association ... See more keywords
Synaptotagmin: Mechanisms of an electrostatic switch
Sign Up to like & getrecommendations! Published in 2020 at "Neuroscience Letters"
DOI: 10.1016/j.neulet.2020.134834
Abstract: Synaptic transmission relies on the fast, synchronous fusion of neurotransmitter filled vesicles with the presynaptic membrane. Synaptotagmin is the Ca2+ sensor that couples the Ca2+ influx into nerve terminals following an action potential with this… read more here.
Keywords: mechanisms electrostatic; synaptotagmin mechanisms; synaptotagmin; electrostatic switch ... See more keywords
Minimal presynaptic protein machinery governing diverse kinetics of calcium-evoked neurotransmitter release
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-54960-1
Abstract: Neurotransmitters are released from synaptic vesicles with remarkable precision in response to presynaptic calcium influx but exhibit significant heterogeneity in exocytosis timing and efficacy based on the recent history of activity. This heterogeneity is critical… read more here.
Keywords: fusion; calcium evoked; calcium; synaptotagmin ... See more keywords
Neurotransmitter release is triggered by a calcium-induced rearrangement in the Synaptotagmin-1/SNARE complex primary interface
Sign Up to like & getrecommendations! Published in 2024 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2409636121
Abstract: Significance The synaptic sensitive factor attachment protein receptor (SNARE) complex and synaptotagmin-1 (Syt1) are required for fast neurotransmitter release. The functions of Syt1 in preparing synaptic vesicles for fusion and executing the triggering step have… read more here.
Keywords: primary interface; snare complex; snare; synaptotagmin ... See more keywords
A lever hypothesis for Synaptotagmin-1 action in neurotransmitter release
Sign Up to like & getrecommendations! Published in 2024 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2417941121
Abstract: Significance Neurotransmitter release requires SNARE complexes that fuse synaptic vesicles with the plasma membrane and the Ca2+-sensor Synaptotagmin-1, which was thought to facilitate membrane fusion directly through its Ca2+-binding loops. However, binding of Synaptotagmin-1 to… read more here.
Keywords: synaptotagmin; neurotransmitter release; snare complexes;
Drosophila studies support a role for a presynaptic synaptotagmin mutation in a human congenital myasthenic syndrome
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0184817
Abstract: During chemical transmission, the function of synaptic proteins must be coordinated to efficiently release neurotransmitter. Synaptotagmin 2, the Ca2+ sensor for fast, synchronized neurotransmitter release at the human neuromuscular junction, has recently been implicated in… read more here.
Keywords: drosophila; synaptotagmin; congenital myasthenic; myasthenic syndrome ... See more keywords