Comparison of actinomycin peptide synthetase formation in Streptomyces chrysomallus and Streptomyces antibioticus
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800244
Abstract: Actinomycin peptide synthetase genes constitute two oppositely oriented transcriptional units, acmADR, and acmBC, separated by a non‐coding intergenic region. Gene constructs of the intergenic region together with its adjoining gene acmA or acmB from the… read more here.
Keywords: peptide synthetase; formation; streptomyces chrysomallus; gene ... See more keywords
Isolation and characterization of cyclic lipopeptides with broad-spectrum antimicrobial activity from Bacillus siamensis JFL15
Sign Up to like & getrecommendations! Published in 2018 at "3 Biotech"
DOI: 10.1007/s13205-018-1443-4
Abstract: In this research, the antimicrobial substance anti-JFL15 was partially purified using a simple two-step extraction process from the cell-free supernatants of Bacillus siamensis JFL15. Anti-JFL15 exhibited a strong antibacterial activity against various multidrug-resistant aquatic bacterial… read more here.
Keywords: bacillus siamensis; siamensis jfl15; jfl15; cyclic lipopeptides ... See more keywords
Corrigendum to "A continuous tyrosyl-tRNA synthetase assay that regenerates the tRNA substrate" [Anal. Biochem. 486 (2015) 86-95].
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2016.11.002
Abstract: The authors regret that in Figure 3 panels B and D, Figure 5 panels B and D, and Figure 6 panel D, kobs (i.e., v0/[Enzyme]) is plotted on the y-axis rather than v0, as indicated… read more here.
Keywords: synthetase assay; trna synthetase; corrigendum continuous; continuous tyrosyl ... See more keywords
A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids
Sign Up to like & getrecommendations! Published in 2020 at "Heliyon"
DOI: 10.1016/j.heliyon.2020.e05140
Abstract: The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids… read more here.
Keywords: amino; amino acids; fluorescent; designed orthogonal ... See more keywords
Symmetric Assembly of a Decameric Subcomplex in Human Multi-tRNA Synthetase Complex Via Interactions between Glutathione Transferase-Homology Domains and Aspartyl-tRNA Synthetase.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2019.08.013
Abstract: Aminoacyl-tRNA synthetases (AARSs) ligate amino acids to their cognate tRNAs during protein synthesis. In humans, eight AARSs and three non-enzymatic AARS-interacting multifunctional proteins (AIMP1-3), which are involved in various biological processes, form a multi-tRNA synthetase… read more here.
Keywords: drs; gst; trna synthetase; synthetase complex ... See more keywords
Novel aspects of glutamine synthetase in ammonia homeostasis
Sign Up to like & getrecommendations! Published in 2020 at "Neurochemistry International"
DOI: 10.1016/j.neuint.2020.104809
Abstract: Elevated blood ammonia (hyperammonemia) is believed to be a major contributor to the neurological sequelae following severe liver disease. Ammonia is cleared via two main mechanisms, the urea cycle pathway and the glutamine synthetase reaction.… read more here.
Keywords: glutamine synthetase; ammonia homeostasis; urea cycle; synthetase reaction ... See more keywords
A case of anti-aminoacyl tRNA synthetase (ARS) antibody-positive polymyositis (PM)/dermatomyositis (DM)-associated interstitial pneumonia (IP) successfully controlled with bosentan therapy
Sign Up to like & getrecommendations! Published in 2017 at "Respiratory Medicine Case Reports"
DOI: 10.1016/j.rmcr.2017.03.021
Abstract: A 72-year-old woman was admitted to our hospital and was diagnosed with interstitial pneumonia (IP) associated with amyopathic dermatomyositis (ADM). The patient experienced three acute IP exacerbations in the 7 years that followed, which were… read more here.
Keywords: ars antibody; antibody positive; bosentan therapy; therapy ... See more keywords
Exploring the Conformational Landscape of a Lanthipeptide Synthetase Using Native Mass Spectrometry.
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DOI: 10.1021/acs.biochem.1c00085
Abstract: Lanthipeptides are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products. These genetically encoded peptides are biosynthesized by multifunctional enzymes (lanthipeptide synthetases) that possess relaxed substrate specificity and catalyze iterative rounds of post-translational modification. Recent… read more here.
Keywords: conformational landscape; native mass; mass spectrometry; lanthipeptide synthetase ... See more keywords
Substrate Recognition Mechanism of a Trichostatin A-Forming Hydroxyamidotransferase.
Sign Up to like & getrecommendations! Published in 2023 at "Biochemistry"
DOI: 10.1021/acs.biochem.3c00025
Abstract: The hydroxyamidotransferase TsnB9 catalyzes hydroxylamine transfer from l-glutamic acid γ-monohydroxamate to the carboxyl group of trichostatic acid to produce the terminal hydroxamic acid group of trichostatin A, which is a potent inhibitor of histone deacetylase… read more here.
Keywords: acid; synthetase; recognition mechanism; hydroxyamidotransferase ... See more keywords
Siderophore Synthetase DesD Catalyzes N-to-C Condensation in Desferrioxamine Biosynthesis.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00167
Abstract: Desferrioxamine siderophores are assembled by the nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase enzyme DesD via ATP-dependent iterative condensation of three N1-hydroxy-N1-succinyl-cadaverine (HSC) units. Current knowledge of NIS enzymology and the desferrioxamine biosynthetic pathway does not account for… read more here.
Keywords: condensation; desd catalyzes; desferrioxamine biosynthesis; desferrioxamine ... See more keywords
FRET monitoring of a nonribosomal peptide synthetase.
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemical biology"
DOI: 10.1038/nchembio.2435
Abstract: Nonribosomal peptide synthetases (NRPSs) are multidomain enzyme templates for the synthesis of bioactive peptides. Large-scale conformational changes during peptide assembly are obvious from crystal structures, yet their dynamics and coupling to catalysis are poorly understood.… read more here.
Keywords: peptide synthetase; fret monitoring; phenylalanine; monitoring nonribosomal ... See more keywords