DNA vaccines: prime time is now
Sign Up to like & getrecommendations! Published in 2020 at "Current Opinion in Immunology"
DOI: 10.1016/j.coi.2020.01.006
Abstract: Recently newer synthetic DNA vaccines have been rapidly advanced to clinical study and have demonstrated an impressive degree of immune potency and tolerability. Improvements in DNA delivery over prior needle and syringe approaches include jet… read more here.
Keywords: vaccines prime; dna; delivery; dna vaccines ... See more keywords
Validation of specific quantitative real-time RT-PCR assay panel for Infectious Bronchitis using synthetic DNA standards and clinical specimens
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2019.113773
Abstract: Abstract Infectious bronchitis (IB) is a highly contagious upper respiratory tract disease of chickens caused by infectious bronchitis virus (IBV), which has various serotypes that do not cross-protect. Vaccine control strategies for this virus are… read more here.
Keywords: real time; quantitative real; time pcr; using synthetic ... See more keywords
Thermo-Programmed Synthetic DNA-Based Receptors
Sign Up to like & getrecommendations! Published in 2023 at "ACS Nano"
DOI: 10.1021/acsnano.2c07039
Abstract: Herein, we present a generalizable and versatile strategy to engineer synthetic DNA ligand-binding devices that can be programmed to load and release a specific ligand at a defined temperature. We do so by re-engineering two… read more here.
Keywords: dna; ligand; based receptors; release ... See more keywords
A new route to synthetic DNA
Sign Up to like & getrecommendations! Published in 2018 at "Nature Biotechnology"
DOI: 10.1038/nbt.4185
Abstract: An enzymatic approach to oligonucleotide synthesis promises to extend the length and fidelity of synthetic DNA read more here.
Keywords: new route; route synthetic; synthetic dna; dna ... See more keywords
Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor perspectives in biology"
DOI: 10.1101/cshperspect.a023812
Abstract: The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes, circuits, and even entire genomes by gene synthesis methods has become an enabling technology for modern molecular biology and enables the design, build,… read more here.
Keywords: dna; synthesis; synthetic biology; biology ... See more keywords
synDNA—a Synthetic DNA Spike-in Method for Absolute Quantification of Shotgun Metagenomic Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "mSystems"
DOI: 10.1128/msystems.00447-22
Abstract: The synDNAs designed in this study enable accurate and reproducible measurements of absolute amount and fold changes of bacterial species in complex microbial communities. The method proposed here is versatile and promising as it can… read more here.
Keywords: dna; shotgun metagenomic; spike; microbial communities ... See more keywords
A phase 1/2A trial of synthetic DNA vaccine immunotherapy targeting HPV-16 and -18 after chemoradiation for cervical cancer.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2018.36.15_suppl.5525
Abstract: 5525Background: DNA immunotherapies targeting HPV oncogenes, E6 and E7, have demonstrated clinical efficacy in pre-invasive cervical lesions. Here, we assessed the feasibility of chemoradiation and... read more here.
Keywords: dna; targeting hpv; trial synthetic; chemoradiation ... See more keywords
A novel synthetic DNA vaccine elicits protective immune responses against Powassan virus
Sign Up to like & getrecommendations! Published in 2020 at "PLoS Neglected Tropical Diseases"
DOI: 10.1371/journal.pntd.0008788
Abstract: Powassan virus (POWV) infection is a tick-borne emerging infectious disease in the United States and North America. Like Zika virus, POWV is a member of the family Flaviviridae. POWV causes severe neurological sequalae, meningitis, encephalitis,… read more here.
Keywords: novel synthetic; synthetic dna; powv sev; powv ... See more keywords
Validation of a Real-Time PCR Assay for Identification of Fresh and Processed Carica papaya Botanical Material: Using Synthetic DNA to Supplement Specificity Evaluation
Sign Up to like & getrecommendations! Published in 2023 at "Foods"
DOI: 10.3390/foods12030530
Abstract: Several commercially important botanicals have a lack of diagnostic testing options that can quickly and unambiguously identify materials of different matrices. Real-time PCR can be a useful, orthogonal approach to identification for its exceptional specificity… read more here.
Keywords: dna; specificity evaluation; time pcr; real time ... See more keywords
Scalable Combinatorial Assembly of Synthetic DNA for Tracking Applications
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24032549
Abstract: Synthetic DNA barcodes are double-stranded DNA molecules designed to carry recoverable information, information that can be used to represent and track objects and organisms. DNA barcodes offer robust, sensitive detection using standard amplification and sequencing… read more here.
Keywords: barcode; dna; generation; design ... See more keywords