Lyophilization of Synthetic Gene Carriers.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9092-4_14
Abstract: Lyophilization, also known as freeze drying, is a widely used method for stabilization, improvement of long-term storage stability, and simplification of handling of drugs and/or carrier systems. Lyophilization is time-consuming and energy-consuming, and hence optimized… read more here.
Keywords: stability; lyophilization synthetic; lyophilization; gene carriers ... See more keywords
Characterizing microRNA-mediated modulation of gene expression noise and its effect on synthetic gene circuits.
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.109573
Abstract: MicroRNAs (miRNAs) have been shown to modulate gene expression noise, but less is known about how miRNAs with different properties may regulate noise differently. Here, we investigate the role of competing RNAs and the composition… read more here.
Keywords: gene expression; noise; expression noise; gene ... See more keywords
Synthetic gene circuits as tools for drug discovery.
Sign Up to like & getrecommendations! Published in 2021 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2021.06.007
Abstract: Within mammalian systems, there exists enormous opportunity to use synthetic gene circuits to enhance phenotype-based drug discovery, to map the molecular origins of disease, and to validate therapeutics in complex cellular systems. While drug discovery… read more here.
Keywords: drug discovery; drug; synthetic gene; gene circuits ... See more keywords
Two-Layered Microfluidic Devices for High-Throughput Dynamic Analysis of Synthetic Gene Circuits in E. coli.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00307
Abstract: Escherichia coli is a common chassis for synthetic gene circuit studies. In addition to the dose-response of synthetic gene circuits, the analysis of dynamic responses is also an important part of the future design of… read more here.
Keywords: synthetic gene; two layered; layered microfluidic; analysis ... See more keywords
A framework for the modular and combinatorial assembly of synthetic gene circuits.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00174
Abstract: Synthetic gene circuits emerge from iterative design-build-test cycles. Most commonly, the time-limiting step is the circuit construction process. Here, we present a hierarchical cloning scheme based on the widespread Gibson assembly method and make the… read more here.
Keywords: circuit; modular combinatorial; gene circuits; combinatorial assembly ... See more keywords
Effect of time delay in a bistable synthetic gene network.
Sign Up to like & getrecommendations! Published in 2021 at "Chaos"
DOI: 10.1063/5.0046373
Abstract: The essence of logical stochastic resonance is the dynamic manipulation of potential wells. The effect of time delay on the depth of potential wells and the width of a bistable region can be inferred by… read more here.
Keywords: effect time; time; potential wells; time delay ... See more keywords
CRISPR-associated type V proteins as a tool for controlling mRNA stability in S. cerevisiae synthetic gene circuits
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1270
Abstract: Abstract Type V-A CRISPR-(d)Cas system has been used in multiplex genome editing and transcription regulation in both eukaryotes and prokaryotes. However, mRNA degradation through the endonuclease activity of Cas12a has never been studied. In this… read more here.
Keywords: mrna stability; mrna degradation; gene circuits; synthetic gene ... See more keywords
Synthetic Gene Circuits: Design, Implement, and Apply
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the IEEE"
DOI: 10.1109/jproc.2021.3134169
Abstract: Synthetic biologists engineered the first genetic toggle switch and clock at the turn of the 21st century. Since the development of these first gene circuits, methods for circuit design and construction have improved dramatically, narrowing… read more here.
Keywords: implement apply; circuits design; synthetic gene; design implement ... See more keywords
Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abj8892
Abstract: The circadian clock regulates tissue homeostasis through temporal control of tissue-specific clock-controlled genes. In articular cartilage, disruptions in the circadian clock are linked to a procatabolic state. In the presence of inflammation, the cartilage circadian… read more here.
Keywords: inflammation; circadian clock; synthetic gene; cartilage ... See more keywords
Synthetic gene circuits take root
Sign Up to like & getrecommendations! Published in 2022 at "Science"
DOI: 10.1126/science.add6805
Abstract: Description Complex spatial patterns of gene expression are engineered in plants to modulate root morphology A long-standing aim of synthetic biology has been to engineer genetic circuits that are able to confer prescribed spatiotemporal patterns… read more here.
Keywords: synthetic gene; circuits take; take root; gene ... See more keywords
A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
Sign Up to like & getrecommendations! Published in 2023 at "Viruses"
DOI: 10.3390/v15051049
Abstract: Porcine deltacoronavirus (PDCoV) is an emergent swine coronavirus which infects cells from the small intestine and induces watery diarrhea, vomiting and dehydration, causing mortality in piglets (>40%). The aim of this study was to evaluate… read more here.
Keywords: synthetic gene; porcine deltacoronavirus; membrane protein; recombinant membrane ... See more keywords