The yeast platform engineered for synthetic gRNA-landing pads enables multiple gene integrations by a single gRNA/Cas9 system.
Sign Up to like & getrecommendations! Published in 2021 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2021.01.011
Abstract: Saccharomyces cerevisiae is a versatile microbial platform to build synthetic metabolic pathways for production of diverse chemicals. To expedite the construction of complex metabolic pathways by multiplex CRISPR-Cas9 genome-edit, eight desirable intergenic loci, located adjacent… read more here.
Keywords: platform; gene integrations; synthetic grna; grna ... See more keywords