SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range.
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DOI: 10.1039/c7cc08740c
Abstract: We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria… read more here.
Keywords: sypher3s genetically; enhanced brightness; probe; genetically encoded ... See more keywords