A tRNA t6A modification system contributes to the sensitivity towards the toxin β-N-methylamino-L-alanine (BMAA) in the cyanobacterium Anabaena sp. PCC 7120.
Sign Up to like & getrecommendations! Published in 2022 at "Aquatic toxicology"
DOI: 10.1016/j.aquatox.2022.106121
Abstract: Cyanobacteria are oxygen-evolving photosynthetic autotrophs essential for nutrient cycling in the environment. They possess multiple control mechanisms for their cellular activities in order to adapt to the environment. While protein translation is essential for cell… read more here.
Keywords: alanine bmaa; cyanobacterium anabaena; bmaa; t6a ... See more keywords
Efficient conversion of N6-threonylcarbamoyladenosine (t6A) into a tRNA native hydantoin cyclic form (ct6A) performed at nucleoside and oligoribonucleotide levels.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc03560h
Abstract: A t6A nucleoside was efficiently and stereospecifically transformed into a hydantoin cyclic form of N6-l-threonylcarbamoyladenosine (ct6A) by the use of polymer bounded carbodiimide (EDC-P) and HOBt. The procedure was successfully applied for a post-synthetic conversion… read more here.
Keywords: threonylcarbamoyladenosine; cyclic form; t6a; ct6a ... See more keywords
Structure of a reaction intermediate mimic in t6A biosynthesis bound in the active site of the TsaBD heterodimer from Escherichia coli
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab026
Abstract: Abstract The tRNA modification N6-threonylcarbamoyladenosine (t6A) is universally conserved in all organisms. In bacteria, the biosynthesis of t6A requires four proteins (TsaBCDE) that catalyze the formation of t6A via the unstable intermediate l-threonylcarbamoyl-adenylate (TC-AMP). While… read more here.
Keywords: heterodimer escherichia; t6a; active site; structure ... See more keywords
Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac056
Abstract: Abstract N 6-Threonylcarbamoyladenosine (t6A) is a universal and pivotal tRNA modification. KEOPS in eukaryotes participates in its biogenesis, whose mutations are connected with Galloway-Mowat syndrome. However, the tRNA substrate selection mechanism by KEOPS and t6A… read more here.
Keywords: trna substrate; t6a biogenesis; trna; t6a ... See more keywords
Specificity in the Biosynthesis of the Universal tRNA Nucleoside N6-Threonylcarbamoyl Adenosine (t6A) - TsaD is the Gatekeeper.
Sign Up to like & getrecommendations! Published in 2020 at "RNA"
DOI: 10.1261/rna.075747.120
Abstract: N6-threonylcarbamoyl adenosine (t6A) is a nucleoside modification found in all kingdoms of life at position 37 of tRNAs decoding ANN codons, which functions in part to restrict translation initiation to AUG and suppress frameshifting at… read more here.
Keywords: adenosine t6a; t6a; tsad; threonylcarbamoyl adenosine ... See more keywords
The structure of the TsaB/TsaD/TsaE complex reveals an unexpected mechanism for the bacterial t6A tRNA-modification.
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.2210/pdb6s84/pdb
Abstract: The universal N6-threonylcarbamoyladenosine (t6A) modification at position A37 of ANN-decoding tRNAs is essential for translational fidelity. In bacteria the TsaC enzyme first synthesizes an l-threonylcarbamoyladenylate (TC-AMP) intermediate. In cooperation with TsaB and TsaE, TsaD then… read more here.
Keywords: structure tsab; tsae; tsab tsad; t6a ... See more keywords
Conservation and Diversification of tRNA t6A-Modifying Enzymes across the Three Domains of Life
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232113600
Abstract: The universal N6-threonylcarbamoyladenosine (t6A) modification occurs at position 37 of tRNAs that decipher codons starting with adenosine. Mechanistically, t6A stabilizes structural configurations of the anticodon stem loop, promotes anticodon–codon pairing and safeguards the translational fidelity.… read more here.
Keywords: trna t6a; life; conservation diversification; t6a ... See more keywords