Mechanism of the oxidative stress‐mediated increase in lipid accumulation by the bacterium, R. opacus PD630: Experimental analysis and genome‐scale metabolic modeling
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27330
Abstract: Appropriate species of oleaginous bacteria, with their high growth rates and lipid accumulation capabilities, can be good contenders for industrial triacylglycerol (TAG) production, compared to microalgae. Further, oxidative stress (OS) can be used to significantly… read more here.
Keywords: accumulation; mechanism; lipid accumulation; increase ... See more keywords
A Light‐Activatable Photocaged Variant of the Ultra‐High Affinity ALFA‐Tag Nanobody
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200079
Abstract: Nanobodies against short linear peptide‐epitopes are widely used to detect and bind proteins of interest (POI) in fusion constructs. Engineered nanobodies that can be controlled by light have found very recent attention for various extra‐… read more here.
Keywords: photocaged variant; affinity; photobody; alfa tag ... See more keywords
The development of an intermediate‐duration tag to characterize the diving behavior of large whales
Sign Up to like & getrecommendations! Published in 2017 at "Ecology and Evolution"
DOI: 10.1002/ece3.2649
Abstract: Abstract The development of high‐resolution archival tag technologies has revolutionized our understanding of diving behavior in marine taxa such as sharks, turtles, and seals during their wide‐ranging movements. However, similar applications for large whales have… read more here.
Keywords: diving behavior; large whales; duration; tag ... See more keywords
DMSO tolerant NAD(P)H recycler enzyme from a pathogenic bacterium, Burkholderia dolosa PC543: effect of N‐/C‐terminal His Tag extension on protein solubility and activity
Sign Up to like & getrecommendations! Published in 2018 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201800036
Abstract: NAD(P)+ dependent formate dehydrogenase (FDH) is an oxidoreductase used as a biocatalyst to regenerate NAD(P)H in reductase‐mediated chiral synthesis reactions. Solvent stability and the need to reduce NADP+ to NADPH, due to the high cost… read more here.
Keywords: solubility activity; dmso tolerant; enzyme; solubility ... See more keywords
Assessing Acoustic Tagging Effects on Survival, Growth, and Swimming Ability of Juvenile Lake Sturgeon
Sign Up to like & getrecommendations! Published in 2019 at "North American Journal of Fisheries Management"
DOI: 10.1002/nafm.10294
Abstract: Acoustic telemetry is used to quantify fish movement, ecology, and habitat use and can contribute to assessing the success of species supplementation. In this field, a better understanding of the effects of tag burden (or… read more here.
Keywords: acoustic tag; lake sturgeon; tag; ecology ... See more keywords
Integrated tandem affinity protein purification using the polyhistidine plus extra 4 amino acids (HiP4) tag system
Sign Up to like & getrecommendations! Published in 2023 at "PROTEOMICS"
DOI: 10.1002/pmic.202200334
Abstract: Peptide tag systems are a robust biophysical and biochemical method that is widely used for protein detection and purification. Here, we developed a novel tag system termed “HiP4” (histidine plus four amino acids) whose epitope… read more here.
Keywords: purification; system; amino acids; hip4 tag ... See more keywords
A Novel Approach for the Production of Aggregation-Prone Proteins Using the Spidroin-Derived NT* Tag.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1859-2_6
Abstract: Spiders have evolved proteins that can be kept in a highly concentrated soluble form in the silk gland yet rapidly assemble into stable silk fibers under certain environmental conditions. The transition between soluble and fibrillar… read more here.
Keywords: aggregation; tag; aggregation prone; production aggregation ... See more keywords
Tag-based information access in image collections: insights from log and eye-gaze analyses
Sign Up to like & getrecommendations! Published in 2019 at "Knowledge and Information Systems"
DOI: 10.1007/s10115-019-01343-4
Abstract: Tag clouds have been utilized as a “social” way to find and visualize information, providing both one-click access and a snapshot of the “aboutness” of a tagged collection. While many research projects have explored and… read more here.
Keywords: information; image; search; tag based ... See more keywords
DGAT1 from the arachidonic-acid-producing microalga Lobosphaera incisa shows late gene expression under nitrogen starvation and substrate promiscuity in a heterologous system
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Applied Phycology"
DOI: 10.1007/s10811-017-1364-3
Abstract: We report the identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1)-encoding gene from the green oleaginous microalga Lobosphaera incisa (SAG 2468), a prolific photosynthetic producer of the n-6 very long chain polyunsaturated fatty acid… read more here.
Keywords: pufa; starvation; incisa; expression ... See more keywords
Assessment of the Validity and Reproducibility of a Novel Standardized Test Meal for the Study of Postprandial Triacylglycerol Concentrations
Sign Up to like & getrecommendations! Published in 2017 at "Lipids"
DOI: 10.1007/s11745-017-4275-9
Abstract: Lipotest® is a standardized fat-rich meal designed for use as a test meal during a fat tolerance test (FTT) for the study of postprandial triacylglycerol (TAG) concentrations. Herein we examined the precision and reproducibility of… read more here.
Keywords: test meal; reproducibility; meal; tag ... See more keywords
Bisphenol S induces obesogenic effects through deregulating lipid metabolism in zebrafish (Danio rerio) larvae.
Sign Up to like & getrecommendations! Published in 2018 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2018.01.163
Abstract: It has been suggested that dramatic increase in obesity may be caused by growing exposure to environmental chemicals. In vitro data has suggested bisphenol S (BPS), a compound widely used in polycarbonate plastic production, can induce… read more here.
Keywords: metabolism; obesogenic effects; zebrafish danio; tag ... See more keywords