A novel nickel-modified nano-magnetite for isolation of histidine-tagged proteins expressed in Escherichia coli
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03637-5
Abstract: Nano-magnetite with superparamagnetism could be coated by some organic compounds or by nano Au or Pt via surface modifications with multi-step reactions for the applications of isolating histidine-tagged (His-tagged) proteins. Introducing active sites of binding… read more here.
Keywords: nano magnetite; escherichia coli; surface; histidine tagged ... See more keywords
Layer by layer coating of NH2-silicate/polycarboxylic acid polymer saturated by Ni2+ onto the super magnetic NiFe2O4 nanoparticles for sensitive and bio-valuable separation of His-tagged proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2017.10.015
Abstract: Magnetic nanoparticles NiFe2O4 was synthesized and covered in the silicate lattice of (3-Aminopropyl) triethoxysilane (APS) by the sol-gel process. Subsequently, the EDTA-dianhydride was attached to the amino surface of magnetic nanoparticles (MNPs) during the nucleophilic… read more here.
Keywords: protein; layer; layer layer; layer coating ... See more keywords
An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc00850c
Abstract: The iminodiacetate-Ni2+-hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization… read more here.
Keywords: polyelectrolyte fluorescent; modified conjugated; iminodiacetate modified; fluorescent labeling ... See more keywords
Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins
Sign Up to like & getrecommendations! Published in 2019 at "SLAS Technology"
DOI: 10.1177/2472630319879647
Abstract: This paper presents a droplet-based immunoassay chip allowing each droplet to be positioned in a passive droplet-positioning cavern under continuous flow. In addition, the chip surface can immobilize any kind of histidine-tagged capture agents for… read more here.
Keywords: multiplex histidine; histidine; histidine tagged; droplet based ... See more keywords
Tools for retargeting proteins within Aspergillus nidulans
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0189077
Abstract: Endogenously tagging proteins with green fluorescent protein (GFP) enables the visualization of the tagged protein using live cell microscopy. GFP-tagging is widely utilized to study biological processes in model experimental organisms including filamentous fungi such… read more here.
Keywords: protein; gbp; gfp tagged; gene ... See more keywords
Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection
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DOI: 10.1371/journal.pone.0191315
Abstract: The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin… read more here.
Keywords: biotin; immobilization; affinity; tag ... See more keywords