Repressor‐Like On‐Off Regulation of Protein Expression by the DNA‐Binding Transcription Activator‐Like Effector in T7 Promoter‐Based Cell‐Free Protein Synthesis
Sign Up to like & getrecommendations! Published in 2020 at "ChemBioChem"
DOI: 10.1002/cbic.202000591
Abstract: The aim of this study was to develop a transcription activator‐like effector (TALE)‐based technology to regulate protein synthesis in cell‐free systems. We attempted to regulate the T7 promoter system, which has no natural mechanism of… read more here.
Keywords: protein; tale; expression; protein synthesis ... See more keywords
Context and number of noncanonical repeat variable diresidues impede the design of TALE proteins with improved DNA targeting
Sign Up to like & getrecommendations! Published in 2019 at "Protein Science"
DOI: 10.1002/pro.3801
Abstract: Transcription activator‐like effector (TALE) proteins have been used extensively for targeted binding of fusion proteins to loci of interest in (epi)genome engineering. Such approaches typically utilize four canonical TALE repeat variable diresidue (RVD) types, corresponding… read more here.
Keywords: tale proteins; tale; repeat variable; improved specificity ... See more keywords
Exploiting the sequence diversity of TALE-like repeats to vary the strength of dTALE-promoter interactions
Sign Up to like & getrecommendations! Published in 2017 at "Synthetic Biology"
DOI: 10.1093/synbio/ysx004
Abstract: Abstract Designer transcription activator-like effectors (dTALEs) are programmable transcription factors used to regulate user-defined promoters. The TALE DNA-binding domain is a tandem series of amino acid repeats that each bind one DNA base. Each repeat… read more here.
Keywords: sequence diversity; tale; repeat; tale like ... See more keywords
An efficient method to clone TAL effector genes from Xanthomonas oryzae using Gibson assembly
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Plant Pathology"
DOI: 10.1111/mpp.12820
Abstract: Summary Transcription Activator‐Like effectors (TALes) represent the largest family of type III effectors among pathogenic bacteria and play a critical role in the process of infection. Strains of Xanthomonas oryzae pv. oryzae (Xoo) and some… read more here.
Keywords: tale genes; tale; xanthomonas oryzae; gibson assembly ... See more keywords
The Hematopoietic TALE-Code Shows Normal Activity of IRX1 in Myeloid Progenitors and Reveals Ectopic Expression of IRX3 and IRX5 in Acute Myeloid Leukemia
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23063192
Abstract: Homeobox genes encode transcription factors that control basic developmental decisions. Knowledge of their hematopoietic activities casts light on normal and malignant immune cell development. Recently, we constructed the so-called lymphoid TALE-code that codifies expression patterns… read more here.
Keywords: cell; tale; myeloid; expression ... See more keywords
Children in the blood jet of poetry : Sylvia Plath's poetic tale of infanticide
Sign Up to like & getrecommendations! Published in 2020 at "Brno studies in English"
DOI: 10.5817/bse2020-1-7
Abstract: The following article presents an analysis of Sylvia Plath’s mature poetry (1959-1962) concerned with the themes of motherhood and children. The analysis rejects the biographical context and instead reads the individual poems through the prism… read more here.
Keywords: children blood; poetry; tale; jet poetry ... See more keywords
Convergent recruitment of TALE homeodomain life cycle regulators to direct sporophyte development in land plants and brown algae
Sign Up to like & getrecommendations! Published in 2019 at "eLife"
DOI: 10.7554/elife.43101
Abstract: Three amino acid loop extension homeodomain transcription factors (TALE HD TFs) act as life cycle regulators in green algae and land plants. In mosses these regulators are required for the deployment of the sporophyte developmental… read more here.
Keywords: tale; sporophyte; cycle regulators; life ... See more keywords