Using Tandem Affinity Purification to Identify Circadian Clock Protein Complexes from Arabidopsis.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1912-4_15
Abstract: Identification of protein-protein interactions is an effective method of elucidating new roles for circadian clock-associated proteins that can expand beyond the information collected from transcriptional studies and genetic screens. Tandem affinity purification coupled with liquid… read more here.
Keywords: protein; tandem affinity; circadian clock; affinity purification ... See more keywords
Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6518-2_2
Abstract: Protein purification by tandem affinity purification (TAP)-tag coupled to mass spectrometry analysis is usually used to reveal protein complex composition. Here we describe a TAP-tag purification of chromatin-bound proteins along with associated nucleosomes, which allow… read more here.
Keywords: tandem affinity; mass spectrometry; chromatin; affinity purification ... See more keywords
Characterization of a functional recombinant human creatine kinase-MB isoenzyme prepared by tandem affinity purification from Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8286-5
Abstract: Creatine kinase isoform CK-MB has been widely applied as a biomarker of myocardial injury. While a variety of methods have been used to measure CK-MB activity or mass in clinical laboratories, a CK-MB standard is… read more here.
Keywords: creatine; tandem affinity; human creatine; affinity purification ... See more keywords
Novel interacting proteins identified by tandem affinity purification coupled to nano LC-MS/MS interact with ribosomal S6 protein kinase 4 (RSK4) and its variant protein (RSK4m).
Sign Up to like & getrecommendations! Published in 2017 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2016.12.030
Abstract: Ribosomal S6 protein kinase 4 (RSK4) is an important novel tumor suppressor that inhibits breast cancer cell growth and induces senescence. However, RSK4m, which is a variant of RSK4 resulting from alternative splicing, may play… read more here.
Keywords: protein; tandem affinity; rsk4 rsk4m; rsk4 ... See more keywords
Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase
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DOI: 10.1186/s13104-018-3500-9
Abstract: AbstractΟbjectiveTo construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag.ResultsWe constructed and tested by transient… read more here.
Keywords: tandem affinity; expression; tag; expression vectors ... See more keywords
Identification of Kv4.2 protein complex and modifications by tandem affinity purification-mass spectrometry in primary neurons
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular Neuroscience"
DOI: 10.3389/fncel.2022.1070305
Abstract: Proteins usually form complexes to fulfill variable physiological functions. In neurons, communication relies on synapses where receptors, channels, and anchoring proteins form complexes to precisely control signal transduction, synaptic integration, and action potential firing. Although… read more here.
Keywords: protein complex; mass spectrometry; affinity purification; tandem affinity ... See more keywords