Downstream Process Intensification for AAV Purification by Affinity Chromatography Using Single Pass Tangential Flow Filtration.
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.70090
Abstract: To enable adeno‐associated viral vectors (AAV) to achieve their maximum potential, next‐generation manufacturing processes must be developed to make gene therapies more affordable and accessible. This study focused on the design of two different intensified… read more here.
Keywords: process; affinity; downstream; scale ... See more keywords
Modeling tangential flow filtration using reverse asymmetric membranes for bioreactor harvesting
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Progress"
DOI: 10.1002/btpr.3084
Abstract: Tangential flow filtration (TFF) has many advantages for bioreactor harvesting, as the permeate could be introduced directly to the subsequent capture step. However, membrane fouling has limited its widespread use. This is particularly problematic given… read more here.
Keywords: tangential flow; filtration; layer; flow filtration ... See more keywords
Evaluation of Single-use Tangential Flow Filtration Technology for Purification of Activated Polysaccharides Used in Conjugate Vaccine Manufacturing.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology progress"
DOI: 10.1002/btpr.3204
Abstract: Over the past decade, single-use tangential flow filtration (TFF) technologies have emerged to reduce system preparation time, promote fast and flexible product change over, and ultimately shorten process development and manufacturing time/cost. In this study,… read more here.
Keywords: use tangential; tangential flow; use; tff ... See more keywords
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3309
Abstract: Robust cell retention devices are key to successful cell culture perfusion. Currently, tangential flow filtration (TFF) and alternating tangential flow filtration (ATF) are most commonly used for this purpose. TFF, however, suffers from poor fouling… read more here.
Keywords: cell; alternating tangential; flow filtration; concept ... See more keywords
Distinct molecular properties and functions of small EV subpopulations isolated from human umbilical cord MSCs using tangential flow filtration combined with size exclusion chromatography
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.70029
Abstract: Abstract As functional derivatives of mesenchymal stem cells (MSCs), small extracellular vesicles (sEVs) have garnered significant attention and application in regenerative medicine. However, the technical limitations for large‐scale isolation of sEVs and their heterogeneous nature… read more here.
Keywords: filtration combined; size; umbilical cord; human umbilical ... See more keywords
Selective protein purification via tangential flow filtration – Exploiting protein-protein complexes to enable size-based separations
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Membrane Science"
DOI: 10.1016/j.memsci.2020.118712
Abstract: Abstract Tangential flow filtration (TFF) is a size-based separation method conventionally used for buffer exchange, concentration, pathogen removal, and for coarse purification. In this study, we use TFF for selective purification of proteins in their… read more here.
Keywords: protein; tangential flow; size based; flow filtration ... See more keywords
Optimized Tangential Flow Filtration for Efficient Isolation of Highly Pure and Bioactive sEVs.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.5c03781
Abstract: Small extracellular vesicles (sEVs) have demonstrated significant therapeutic potential. However, the lack of efficient and scalable methods for sEV separation remains a critical barrier to their large-scale production as therapeutic agents. Tangential flow filtration (TFF)… read more here.
Keywords: tff parameters; filtration; flow filtration; tangential flow ... See more keywords
Separation of sago starch from model suspensions by tangential flow filtration
Sign Up to like & getrecommendations! Published in 2018 at "Chemical Engineering Communications"
DOI: 10.1080/00986445.2018.1544897
Abstract: Abstract Sago starch producing mills in Malaysia generate approximately 20 tons of effluent per ton of starch produced. The effluent contains mainly starch and very low concentrations of nitrogenous compounds. The starch could be recoverable… read more here.
Keywords: tangential flow; sago starch; filtration; flow filtration ... See more keywords
Harnessing 3D cultured MSC exosomes through tangential flow filtration for enhanced diabetic wound healing
Sign Up to like & getrecommendations! Published in 2025 at "Stem Cells Translational Medicine"
DOI: 10.1093/stcltm/szaf064
Abstract: Abstract Introduction Mesenchymal stem cell-derived exosomes have garnered considerable attention in regenerative medicine due to their non-immunogenicity, low infusion toxicity, easy accessibility, straightforward preservation, and minimal ethical concerns. While ultracentrifugation is the prevailing method for… read more here.
Keywords: diabetic wound; wound healing; ultracentrifugation; flow filtration ... See more keywords
Evaluation of tangential flow filtration coupled to long-read sequencing for ostreid herpesvirus type 1 genome assembly
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Genomics"
DOI: 10.1099/mgen.0.000895
Abstract: Whole-genome sequencing is widely used to better understand the transmission dynamics, the evolution and the emergence of new variants of viral pathogens. This can bring crucial information to stakeholders for disease management. Unfortunately, aquatic virus… read more here.
Keywords: ostreid herpesvirus; flow filtration; genome; herpesvirus type ... See more keywords
Tangential flow filtration facilitated washing of human red blood cells: A proof‐of‐concept study
Sign Up to like & getrecommendations! Published in 2022 at "Vox Sanguinis"
DOI: 10.1111/vox.13259
Abstract: Red blood cell (RBC) units in hypothermic storage degrade over time, commonly known as the RBC storage lesion. These older RBC units can cause adverse clinical effects when transfused, as older RBCs in the unit… read more here.
Keywords: study; red blood; flow filtration; tangential flow ... See more keywords