A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli
Sign Up to like & getrecommendations! Published in 2020 at "Archives of Microbiology"
DOI: 10.1007/s00203-020-01860-9
Abstract: Polymerases are enzymes that synthesize long chains or polymers of nucleic acids including DNA or RNA from nucleotides. They assemble nucleic acids by copying a DNA or RNA template strand using base-pairing interactions. One of… read more here.
Keywords: biology; dna polymerase; taq dna; dna ... See more keywords
Oligoribonucleotide-Mediated Blockade of DNA Extension by Taq DNA Polymerases Increases Specificity and Sensitivity for Detecting Single-Nucleotide Differences.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05186
Abstract: Blocking PCR is a method that inhibits amplification of DNA possessing a nucleotide sequence complementary to that of a blocker; the method can be used to suppress amplification of target wild-type DNA while amplifying mutated… read more here.
Keywords: dna; extension; taq dna; dna polymerases ... See more keywords
Cyclization of secondarily structured oligonucleotides to single-stranded rings by using Taq DNA ligase at high temperatures
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DOI: 10.1039/c8ra02804d
Abstract: Single-stranded DNA rings play important roles in nanoarchitectures, molecular machines, DNA detection, etc. Although T4 DNA ligase has been widely employed to cyclize single-stranded oligonucleotides into rings, the cyclization efficiency is very low when the… read more here.
Keywords: single stranded; dna; taq dna; secondarily structured ... See more keywords
Single-molecule Taq DNA polymerase dynamics
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abl3522
Abstract: Taq DNA polymerase functions at elevated temperatures with fast conformational dynamics—regimes previously inaccessible to mechanistic, single-molecule studies. Here, single-walled carbon nanotube transistors recorded the motions of Taq molecules processing matched or mismatched template–deoxynucleotide triphosphate pairs… read more here.
Keywords: single molecule; taq dna; dna polymerase;
Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization
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DOI: 10.1371/journal.pone.0184162
Abstract: DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR.… read more here.
Keywords: protein; dna; dna polymerase; taq dna ... See more keywords
Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2023.1180542
Abstract: Because of its non-template addition feature, Taq DNA polymerase can catalyze one or more extra nucleotides onto the 3′ terminus of PCR products. An extra peak is observed at DYS391 locus after the PCR products… read more here.
Keywords: addition; taq dna; pcr; dna polymerase ... See more keywords