A newly developed BVDV-1 RT-qPCR Taqman assay based on Italian isolates: evaluation as a diagnostic tool
Sign Up to like & getrecommendations! Published in 2017 at "Folia Microbiologica"
DOI: 10.1007/s12223-017-0497-8
Abstract: A single-step TaqMan® RT-qPCR was developed for the detection of bovine viral diarrhea virus type 1 (BVDV-1), an important pathogen of cattle worldwide. The assay was based on conserved 5’UTR sequences of Italian BVDV-1 isolates.… read more here.
Keywords: qpcr; taqman; assay based; bvdv ... See more keywords
Taqman Assay for Genotyping CKD-Associated APOL1 SNP rs60910145: A Cautionary Note
Sign Up to like & getrecommendations! Published in 2019 at "Kidney International Reports"
DOI: 10.1016/j.ekir.2018.09.018
Abstract: When combined with results from the rs73885319 and rs71785313 SNP assays, will result in an inferred haplotype not observed on African chromosomes. To the Editor: There are 2 APOL1 variant haplotypes, G1 and G2, that… read more here.
Keywords: taqman; base pair; haplotype; snp ... See more keywords
Development and evaluation of a novel loop mediated isothermal amplification coupled with TaqMan probe assay for detection of genetically modified organism with NOS terminator.
Sign Up to like & getrecommendations! Published in 2021 at "Food chemistry"
DOI: 10.1016/j.foodchem.2021.129684
Abstract: In this study, we aim to develop a novel loop mediated isothermal amplification (LAMP) coupled with TaqMan (LAMP-TaqMan) method for quick qualitative detection of genetically modified organism (GMOs). We designed four LAMP primers and one… read more here.
Keywords: loop mediated; detection; novel loop; taqman ... See more keywords
Rapid differential detection of Genotype I and III Japanese Encephalitis Virus from clinical samples by a novel duplex TaqMan probe-based RT-qPCR assay.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2020.113841
Abstract: Japanese Encephalitis (JE) is an acute infectious disease that threatens both human and pig populations throughout Asia. JE is caused by the Japanese Encephalitis Virus (JEV), of which Genotype III (GIII) had been the most… read more here.
Keywords: jev; taqman; japanese encephalitis; duplex taqman ... See more keywords
Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2017.04.005
Abstract: Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan®-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on… read more here.
Keywords: ns1 gene; taqman; assessment ns1; pcr detection ... See more keywords
Two new superior primer pairs for universal detection of Xylella spp. in conventional PCR and TaqMan quantitative real-time PCR.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106321
Abstract: X. fastidiosa causes many economically important plant diseases such as Pierce's disease of grapevine, citrus variegated chlorosis disease, and olive quick decline syndrome. Another species in the same genus, Xylella taiwanensis, causes pear leaf scorch.… read more here.
Keywords: spp conventional; taqman; pcr; primer pairs ... See more keywords
Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1101/2022.06.23.497358
Abstract: Herein, we developed a single and a duplex TaqMan quantitative qPCR for absolute quantification of copy numbers of integrated dihydrofolate reductase-thymidylate synthase (mdhfr-ts) drug selectable marker for pyrimethamine resistance in Toxoplasma gondii knockouts (KOs). The… read more here.
Keywords: crispr cas9; duplex taqman; taqman; toxoplasma ... See more keywords
Development and evaluation of a TaqMan MGB RT-PCR assay for detection of H5 and N8 subtype influenza virus
Sign Up to like & getrecommendations! Published in 2020 at "BMC Infectious Diseases"
DOI: 10.1186/s12879-020-05277-z
Abstract: Background Highly pathogenic influenza A (H5N8) viruses have caused several worldwide outbreaks in birds and are of potential risk to humans. Thus, a specific, rapid and sensitive method for detection is urgently needed. Methods In… read more here.
Keywords: avian influenza; detection; influenza viruses; taqman ... See more keywords
Partial validation of a TaqMan quantitative polymerase chain reaction for the detection of the three genotypes of Infectious spleen and kidney necrosis virus
Sign Up to like & getrecommendations! Published in 2023 at "PLOS ONE"
DOI: 10.1371/journal.pone.0281292
Abstract: Megalocytiviruses (MCVs) are double-stranded DNA viruses known to infect important freshwater and marine fish species in the aquaculture, food, and ornamental fish industries worldwide. Infectious spleen and kidney necrosis virus (ISKNV) is the type species… read more here.
Keywords: taqman; infectious spleen; three genotypes; detection ... See more keywords