Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
Sign Up to like & getrecommendations! Published in 2022 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-022-11822-4
Abstract: Abstract The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the… read more here.
Keywords: sybr green; primer sets; sars cov; based qpcr ... See more keywords
Development of a TaqMan-based real-time PCR for detecting duck adenovirus 3.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2018.08.011
Abstract: Recently, a novel duck adenovirus (designated as duck adenovirus 3, DAdV-3) was discovered in Muscovy ducks, China. Here, we developed a TaqMan-based real-time PCR assay (qPCR) for the detection of DAdV-3 infection. After the optimization… read more here.
Keywords: real time; duck; duck adenovirus; taqman based ... See more keywords
TaqMan-based real-time polymerase chain reaction assay for specific detection of bocavirus-1 in domestic cats
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and Cellular Probes"
DOI: 10.1016/j.mcp.2020.101647
Abstract: Abstract Feline bocavirus-1 (FBoV-1) was first discovered in Hong Kong in 2012, and studies have indicated that the virus may cause feline hemorrhagic enteritis. Currently, there is a lack of an effective and quantitative method… read more here.
Keywords: detection; real time; bocavirus; taqman based ... See more keywords
Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen
Sign Up to like & getrecommendations! Published in 2020 at "BioMed Research International"
DOI: 10.1155/2020/5673145
Abstract: This study described a TaqMan based real-time fluorescent quantitative PCR (qPCR) method to detect porcine cytomegalovirus (PCMV) infection, targeting the conserved region of the DNA polymerase (DPOL) gene. The standard curve showed a linear regression… read more here.
Keywords: real time; time fluorescent; porcine; taqman based ... See more keywords