TaqMan probes as blocking agents for enriched PCR amplification and DNA melting analysis of mutant genes.
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DOI: 10.2144/000114515
Abstract: Asymmetric PCR and DNA melting analysis with TaqMan probes applied for mutation detection is effectively used in clinical diagnostics. The method is simple, cost-effective, and carried out in a closed-tube format, minimizing time, labor, and… read more here.
Keywords: dna melting; blocking agents; taqman probes; melting analysis ... See more keywords