Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2019.01.015
Abstract: Mixed infections with different pathogens are common in sheep and goats under intensive production conditions. Quick and accurate detection and differentiation of different pathogens is necessary for epidemiological surveillance, disease management and import and export… read more here.
Keywords: taqman qpcr; multiplex taqman; sheep goats; detection differentiation ... See more keywords
A triplex probe-based TaqMan qPCR assay for Calreticulin type I and II mutation detection
Sign Up to like & getrecommendations! Published in 2019 at "Hematology"
DOI: 10.1080/10245332.2018.1502944
Abstract: ABSTRACT Background: Calreticulin (CALR) exon 9 frameshift mutations have recently been identified in 30–40% of patients with essential thrombocythemia (ET) and primary myelofibrosis (PMF) without JAK2 or MPL mutations. We aimed to develop a qPCR… read more here.
Keywords: taqman qpcr; probe based; triplex probe; type ... See more keywords
A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species
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DOI: 10.1371/journal.pone.0177808
Abstract: Rubus stunt is an economically important disease in the production of raspberries, blackberries, and loganberries. A fast, sensitive, and reliable diagnosis of phytoplasmas, the causal agent of the disease, is of prime importance to stop… read more here.
Keywords: phytoplasmas; taqman qpcr; detection phytoplasmas; qpcr assay ... See more keywords
Construction of Infectious cDNA Clone of Brassica Yellows Virus Isolated from Strawberry and Establishment of TaqMan RT-qPCR
Sign Up to like & getrecommendations! Published in 2022 at "Plants"
DOI: 10.3390/plants11233380
Abstract: The natural host range for brassica yellows virus (BrYV) is generally limited to Cruciferae. However, we found that BrYV can naturally infect strawberry. The full-length genome sequences of BrYV-MB (accession No. MZ666129) and BrYV-HY (accession… read more here.
Keywords: taqman qpcr; cdna clone; infectious cdna; yellows virus ... See more keywords