Amide Modifications in the Seed Region of the Guide Strand Improve the On-Target Specificity of Short Interfering RNA.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00769
Abstract: RNA interference (RNAi) is a well-established research tool and is also maturing as a novel therapeutic approach. For the latter, microRNA-like off-target activity of short interfering RNAs (siRNAs) remains as one of the main problems… read more here.
Keywords: short interfering; guide strand; seed; target activity ... See more keywords
Amide Internucleoside Linkages Suppress the MicroRNA-like Off-Target Activity of Short Interfering RNA.
Sign Up to like & getrecommendations! Published in 2025 at "ACS chemical biology"
DOI: 10.1021/acschembio.4c00824
Abstract: RNA interference (RNAi) has rapidly matured as a novel therapeutic approach. In this field, chemical modifications have been critical to the clinical success of short interfering RNAs (siRNAs). Notwithstanding the significant advances, achieving robust durability… read more here.
Keywords: microrna like; like target; target activity; target ... See more keywords
Optimizing genome editing strategy by primer-extension-mediated sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Cell Discovery"
DOI: 10.1038/s41421-019-0088-8
Abstract: Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing… read more here.
Keywords: genome editing; mediated sequencing; extension mediated; target ... See more keywords
DeepMEns: an ensemble model for predicting sgRNA on-target activity based on multiple features
Sign Up to like & getrecommendations! Published in 2024 at "Briefings in Functional Genomics"
DOI: 10.1093/bfgp/elae043
Abstract: Abstract The CRISPR/Cas9 system developed from Streptococcus pyogenes (SpCas9) has high potential in gene editing. However, its successful application is hindered by the considerable variability in target efficiencies across different single guide RNAs (sgRNAs). Although… read more here.
Keywords: sgrna target; part uses; target activity; target ... See more keywords
Global quantification exposes abundant low-level off-target activity by base editors.
Sign Up to like & getrecommendations! Published in 2021 at "Genome research"
DOI: 10.1101/gr.275770.121
Abstract: Base editors are dedicated engineered deaminases that enable directed conversion of specific bases in the genome or transcriptome in a precise and efficient manner, and hold promise for correcting pathogenic mutations. A major concern limiting… read more here.
Keywords: base editors; genome transcriptome; target; base ... See more keywords
Cas9 endonuclease and off-target activity
Sign Up to like & getrecommendations! Published in 2017 at "Science"
DOI: 10.1126/science.357.6350.467-q
Abstract: Genome Sequencing The RNA-guided endonuclease Cas9 has brought about a revolution in genetic engineering and genome editing. However, issues with the system include the off-target activity of the endonuclease and promiscuous cleavage, making accuracy in… read more here.
Keywords: cas9 endonuclease; endonuclease; target activity; endonuclease target ... See more keywords
Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases.
Sign Up to like & getrecommendations! Published in 2019 at "Annual review of biochemistry"
DOI: 10.1146/annurev-biochem-013118-111730
Abstract: Programmable nucleases and deaminases, which include zinc-finger nucleases, transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA-guided base editors, are now widely employed for the targeted modification of genomes in cells and organisms. These gene-editing… read more here.
Keywords: target activity; gene editing; nucleases deaminases; target ... See more keywords
CRISPRpred: A flexible and efficient tool for sgRNAs on-target activity prediction in CRISPR/Cas9 systems
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0181943
Abstract: The CRISPR/Cas9-sgRNA system has recently become a popular tool for genome editing and a very hot topic in the field of medical research. In this system, Cas9 protein is directed to a desired location for… read more here.
Keywords: crispr cas9; sgrnas target; target activity; tool ... See more keywords
SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0231716
Abstract: RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce… read more here.
Keywords: activity; target; directed evolution; evolution cas9 ... See more keywords