Amide Modifications in the Seed Region of the Guide Strand Improve the On-Target Specificity of Short Interfering RNA.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00769
Abstract: RNA interference (RNAi) is a well-established research tool and is also maturing as a novel therapeutic approach. For the latter, microRNA-like off-target activity of short interfering RNAs (siRNAs) remains as one of the main problems… read more here.
Keywords: short interfering; guide strand; seed; target activity ... See more keywords
Optimizing genome editing strategy by primer-extension-mediated sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Cell Discovery"
DOI: 10.1038/s41421-019-0088-8
Abstract: Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing… read more here.
Keywords: genome editing; mediated sequencing; extension mediated; target ... See more keywords
Global quantification exposes abundant low-level off-target activity by base editors.
Sign Up to like & getrecommendations! Published in 2021 at "Genome research"
DOI: 10.1101/gr.275770.121
Abstract: Base editors are dedicated engineered deaminases that enable directed conversion of specific bases in the genome or transcriptome in a precise and efficient manner, and hold promise for correcting pathogenic mutations. A major concern limiting… read more here.
Keywords: base editors; genome transcriptome; target; base ... See more keywords
Cas9 endonuclease and off-target activity
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DOI: 10.1126/science.357.6350.467-q
Abstract: Genome Sequencing The RNA-guided endonuclease Cas9 has brought about a revolution in genetic engineering and genome editing. However, issues with the system include the off-target activity of the endonuclease and promiscuous cleavage, making accuracy in… read more here.
Keywords: cas9 endonuclease; endonuclease; target activity; endonuclease target ... See more keywords
Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases.
Sign Up to like & getrecommendations! Published in 2019 at "Annual review of biochemistry"
DOI: 10.1146/annurev-biochem-013118-111730
Abstract: Programmable nucleases and deaminases, which include zinc-finger nucleases, transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA-guided base editors, are now widely employed for the targeted modification of genomes in cells and organisms. These gene-editing… read more here.
Keywords: target activity; gene editing; nucleases deaminases; target ... See more keywords
CRISPRpred: A flexible and efficient tool for sgRNAs on-target activity prediction in CRISPR/Cas9 systems
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0181943
Abstract: The CRISPR/Cas9-sgRNA system has recently become a popular tool for genome editing and a very hot topic in the field of medical research. In this system, Cas9 protein is directed to a desired location for… read more here.
Keywords: crispr cas9; sgrnas target; target activity; tool ... See more keywords
SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0231716
Abstract: RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce… read more here.
Keywords: activity; target; directed evolution; evolution cas9 ... See more keywords