Whole‐genome sequencing of SARS‐CoV‐2: Comparison of target capture and amplicon single molecule real‐time sequencing protocols
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28123
Abstract: Fast, accurate sequencing methods are needed to identify new variants and genetic mutations of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) genome. Single‐molecule real‐time (SMRT) Pacific Biosciences (PacBio) provides long, highly accurate sequences by… read more here.
Keywords: amplicon; target; target capture; protocol ... See more keywords
Whole‐genome single molecule real‐time sequencing of SARS‐CoV‐2 Omicron
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28564
Abstract: New variants and genetic mutations of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) genome can only be identified using accurate sequencing methods. Single molecule real‐time (SMRT) sequencing has been used to characterize Alpha and… read more here.
Keywords: target capture; cov omicron; whole genome; protocol ... See more keywords
Target capture sequencing of SARS-CoV-2 genomes using the ONETest Coronaviruses Plus
Sign Up to like & getrecommendations! Published in 2021 at "Diagnostic Microbiology and Infectious Disease"
DOI: 10.1016/j.diagmicrobio.2021.115508
Abstract: We introduce a target capture next-generation sequencing methodology, the ONETest Coronaviruses Plus, to sequence SARS-CoV-2 genomes and select loci of other respiratory viruses. We applied the ONETest on 70 respiratory samples (collected in Florida, USA… read more here.
Keywords: coronaviruses plus; sars cov; onetest coronaviruses; target capture ... See more keywords
Phylogenetics of moth-like butterflies (Papilionoidea: Hedylidae) based on a new 13-locus target capture probe set.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular phylogenetics and evolution"
DOI: 10.1016/j.ympev.2018.06.002
Abstract: The Neotropical moth-like butterflies (Hedylidae) are perhaps the most unusual butterfly family. In addition to being species-poor, this family is predominantly nocturnal and has anti-bat ultrasound hearing organs. Evolutionary relationships among the 36 described species… read more here.
Keywords: target capture; probe; moth like; probe set ... See more keywords
Efficient target capture and transport by fuel-free micromotors in a multichannel microchip.
Sign Up to like & getrecommendations! Published in 2017 at "Soft matter"
DOI: 10.1039/c7sm01905j
Abstract: Efficient capture and transport of biological targets by functionalized micromotors in microfluidic chips have emerged as to be promising for bioanalysis and detection of targets. However, the crucial step-target capture-is still inefficient due to the… read more here.
Keywords: capture transport; target capture; target; multichannel microchip ... See more keywords
Using target capture to address conservation challenges: population-level tracking of a globally-traded herbal medicine.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular ecology resources"
DOI: 10.1111/1755-0998.13472
Abstract: The promotion of responsible and sustainable trade in biological resources is widely proposed as one solution to mitigate currently high levels of global biodiversity loss. Various molecular identification methods have been proposed as appropriate tools… read more here.
Keywords: medicine; conservation; plant; target capture ... See more keywords
Taxon-specific or universal? Using target capture to study the evolutionary history of rapid radiations.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular ecology resources"
DOI: 10.1111/1755-0998.13523
Abstract: Target capture has emerged as an important tool for phylogenetics and population genetics in non-model taxa. Whereas developing taxon-specific capture probes requires sustained efforts, available universal kits may have a lower power to reconstruct relationships… read more here.
Keywords: taxon specific; capture; study evolutionary; target capture ... See more keywords
Target-capture probes for phylogenomics of the Caenogastropoda.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular ecology resources"
DOI: 10.1111/1755-0998.13793
Abstract: Target-capture approaches have facilitated a rapid growth in the field of phylogenomics but few probe sets exist for mollusks, an exceptionally rich phylum with unparalleled ecological and morphological diversity. We designed and tested the first… read more here.
Keywords: probe set; loci; target capture; probe ... See more keywords
A novel assembly pipeline and functional annotations for targeted sequencing: A case study on the globally threatened Margaritiferidae (Bivalvia: Unionida).
Sign Up to like & getrecommendations! Published in 2023 at "Molecular ecology resources"
DOI: 10.1111/1755-0998.13802
Abstract: The proliferation of genomic sequencing approaches has significantly impacted the field of phylogenetics. Target capture approaches provide a cost-effective, fast and easily applied strategy for phylogenetic inference of non-model organisms. However, several existing target capture… read more here.
Keywords: new pipeline; target capture; functional annotations; novel assembly ... See more keywords
Target capture next‐generation sequencing in non‐inversion haemophilia: an alternative approach
Sign Up to like & getrecommendations! Published in 2020 at "British Journal of Haematology"
DOI: 10.1111/bjh.16584
Abstract: ter DW. Refractory autoimmune hemolytic anemia after intestinal transplant responding to conversion from a calcineurin to mTOR inhibitor. Pediatr Transplant. 2013;17:466–71. 13. Botija G, Ybarra M, Ramos E, Molina M, Sarria J, Martinez-Ojinaga E, et… read more here.
Keywords: target capture; transplant; capture next; lymphoproliferative disorder ... See more keywords
Abstract 425: Linked Target Capture: Rapid and high-performance NGS target enrichment for clinical sequencing applications
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Research"
DOI: 10.1158/1538-7445.am2018-425
Abstract: With the continued decrease of DNA sequencing costs, targeted next-generation sequencing (NGS) is seeing broad adoption in many research, commercial and clinical settings. However, current target capture methods typically have long, multiday workflows that can… read more here.
Keywords: dna; target capture; target; capture ... See more keywords