Immobilization-Free Electrochemical DNA Sensor based on signal cascade amplification strategy.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.2174
Abstract: The development of convenient and efficient strategies without using complex nanomaterials or enzymes for signal amplification is very important for bioanalytical applications. Herein, a novel electrochemical DNA sensor was developed by harnessing the signal amplification… read more here.
Keywords: target dna; dna; electrochemical dna; dna sensor ... See more keywords
Identification of Mycoplasma pneumoniae by DNA‐modified gold nanomaterials in a colorimetric assay
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.2377
Abstract: Mycoplasma pneumoniae is a highly infectious bacterium and the major cause of pneumonia especially in school‐going children. Mycoplasma pneumoniae affects the respiratory tract, and 25% of patients experience health‐related problems. It is important to have… read more here.
Keywords: dna; pneumoniae; gnps; target dna ... See more keywords
Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS Method.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0687-2_15
Abstract: Clustered regularly interspaced palindromic repeat (CRISPR) systems are revolutionizing many areas of biology and medicine, where they are increasingly utilized as therapeutic tools for correcting disease-causing mutations. From a clinical perspective, unintended off-target (OT) DNA… read more here.
Keywords: bliss method; genome wide; target dna; crispr ... See more keywords
Transcriptome and target DNA enrichment sequence data provide new insights into the phylogeny of vespid wasps (Hymenoptera: Aculeata: Vespidae).
Sign Up to like & getrecommendations! Published in 2017 at "Molecular phylogenetics and evolution"
DOI: 10.1016/j.ympev.2017.08.020
Abstract: The wasp family Vespidae comprises more than 5000 described species which represent life history strategies ranging from solitary and presocial to eusocial and socially parasitic. The phylogenetic relationships of the major vespid wasp lineages (i.e.,… read more here.
Keywords: vespidae; target dna; vespid wasps; sequence data ... See more keywords
Quantification of mRNA in Single Cells Based on Dimerization-Induced Photoluminescence Nonblinking of Quantum Dots.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02209
Abstract: Photoluminescence (PL) intermittency (or "blinking") is a unique characteristic of single quantum dot (QD) emission. Here, we report a novel single-molecule detection strategy for the intracellular mRNA of interest using the mRNA-induced nonblinking QD dimers… read more here.
Keywords: photoluminescence; dna; mrna single; target dna ... See more keywords
Noninvasive Prognosis of Postmyocardial Infarction Using Urinary miRNA Ultratrace Detection Based on Single-Target DNA-Functionalized AuNPs.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.1c17883
Abstract: Urine is the most appropriate body fluid for analysis because it is easily and less-invasively obtained than blood; thus, urinary miRNAs can better represent the local stage of the disease and might grow up to… read more here.
Keywords: urinary mirna; dna; single target; target dna ... See more keywords
Dynamics of Target DNA Binding and Cleavage by Staphylococcus aureus Cas9 as Revealed by High-Speed Atomic Force Microscopy.
Sign Up to like & getrecommendations! Published in 2023 at "ACS nano"
DOI: 10.1021/acsnano.2c10709
Abstract: Programmable DNA binding and cleavage by CRISPR-Cas9 has revolutionized the life sciences. However, the off-target cleavage observed in DNA sequences with some homology to the target still represents a major limitation for a more widespread… read more here.
Keywords: cleavage; microscopy; dna binding; cas9 ... See more keywords
CRISPR-Cas12a-assisted nucleic acid detection
Sign Up to like & getrecommendations! Published in 2018 at "Cell Discovery"
DOI: 10.1038/s41421-018-0028-z
Abstract: Dear Editor, Today, the need for time-effective and cost-effective nucleic acid detection methods is still growing in fields such as human genotyping and pathogen detection. Using synthetic biomolecular components, many methods have been developed for… read more here.
Keywords: target dna; detection; crispr; cas12a ... See more keywords
The Fabrication of 2D Cu-Based MOF Nanosheets for DNA Detection
Sign Up to like & getrecommendations! Published in 2019 at "Australian Journal of Chemistry"
DOI: 10.1071/ch19312
Abstract: The Cu-based metal–organic framework (MOF) analogues, copper 1,4-benzenedicarboxylate (CuBDC), copper 2,6-naphthalenedicarboxylate (Cu(2,6-NDC)), and copper 1,4-naphthalenedicarboxylate (Cu(1,4-NDC)) MOF nanosheets, are prepared as biosensor nanoplatforms for DNA detection by a spray method. With the ultrathin 2D structure,… read more here.
Keywords: target dna; detection; mof nanosheets; fabrication based ... See more keywords
Comparison of DNA fragments as donor DNAs upon sequence conversion of cleaved target DNA
Sign Up to like & getrecommendations! Published in 2017 at "Nucleosides, Nucleotides and Nucleic Acids"
DOI: 10.1080/15257770.2017.1310385
Abstract: ABSTRACT Pinpoint sequence alteration (genome editing) by the combination of the site-specific cleavage of a target DNA and a donor nucleic acid has attracted much attention and the sequence of the target DNA is expected… read more here.
Keywords: target dna; dna; sequence conversion; sequence ... See more keywords
Structural insights into target DNA recognition and cleavage by the CRISPR-Cas12c1 system
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac987
Abstract: Abstract Cas12c is the recently characterized dual RNA-guided DNase effector of type V-C CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein) systems. Due to minimal requirements for a protospacer adjacent motif (PAM), Cas12c… read more here.
Keywords: cleavage; crispr; target dna; cas12c1 ... See more keywords