CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.201902312
Abstract: Abstract Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) systems, which make the targeted modification of… read more here.
Keywords: genome editing; target effects; crispr cas; target ... See more keywords
Off-target effects of base editors: what we know and how we can reduce it.
Sign Up to like & getrecommendations! Published in 2021 at "Current genetics"
DOI: 10.1007/s00294-021-01211-1
Abstract: The recently discovered CRISPR-Cas9 modification, base editors (BEs), is considered as one of the most promising tools for correcting disease-causing mutations in humans, since it allows point substitutions to be edited without generating double-stranded DNA… read more here.
Keywords: target effects; effects base; genetics; base editors ... See more keywords
Off-target genome editing: A new discipline of gene science and a new class of medicine
Sign Up to like & getrecommendations! Published in 2019 at "Cell Biology and Toxicology"
DOI: 10.1007/s10565-019-09475-7
Abstract: With an increasing growth of genome editing, off-target effects such as non-specific genetic modifications resulting from the designed process of genome editing become a new discipline of gene science and new class medicine. The degree… read more here.
Keywords: genome editing; medicine; target effects; edited cells ... See more keywords
Mosquito control with green nanopesticides: towards the One Health approach? A review of non-target effects
Sign Up to like & getrecommendations! Published in 2017 at "Environmental Science and Pollution Research"
DOI: 10.1007/s11356-017-9752-4
Abstract: The rapid spread of highly aggressive arboviruses, parasites, and bacteria along with the development of resistance in the pathogens and parasites, as well as in their arthropod vectors, represents a huge challenge in modern parasitology… read more here.
Keywords: target effects; control; control green; non target ... See more keywords
Non-target effects of Metarhizium brunneum (BIPESCO 5/F 52) in soil show that this fungus varies between being compatible with, or moderately harmful to, four predatory arthropods
Sign Up to like & getrecommendations! Published in 2019 at "Biological Control"
DOI: 10.1016/j.biocontrol.2019.01.002
Abstract: Abstract Biological control with entomopathogenic fungi is a feasible option for regulation of pest insect populations. However, possible effects on beneficial arthropods must be considered. We assessed the non-target effects of the microbial biological control… read more here.
Keywords: target effects; predatory; non target; soil ... See more keywords
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.107689
Abstract: CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence… read more here.
Keywords: target effects; crispr editing; detection deleterious; target ... See more keywords
What Are the Off-Target Effects of Plan “S” For Translational Investigators?
Sign Up to like & getrecommendations! Published in 2019 at "JACC: Basic to Translational Science"
DOI: 10.1016/j.jacbts.2019.01.005
Abstract: “There are known knowns; there are things we know we know. We also know there are known unknowns; that is to say we know there are some things we do not know. But there are… read more here.
Keywords: effects plan; target effects; plan translational; translational investigators ... See more keywords
Non-target effects of agri-environmental schemes on solitary bees and fungi in the United Kingdom
Sign Up to like & getrecommendations! Published in 2022 at "Bulletin of Entomological Research"
DOI: 10.1017/s0007485322000414
Abstract: Abstract Agri-environmental schemes (AES) are used to enhance pollinator diversity on agricultural farms within the UK. Though the impacts of these schemes on archetypal pollinator species such as the bumblebee (Bombus) and honeybee (Apis) are… read more here.
Keywords: agri environmental; target effects; solitary bees; bee ... See more keywords
Chemical Modification of the siRNA Seed Region Suppresses Off-Target Effects by Steric Hindrance to Base-Pairing with Targets
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DOI: 10.1021/acsomega.7b00291
Abstract: Chemical modifications of 2′-O-methyl (2′-OMe) and locked nucleic acid (LNA) of the nucleotides in the seed region (positions 2–8) of the small interfering RNA (siRNA) guide strand significantly reduced seed-matched (SM) off-target effects. The siRNA… read more here.
Keywords: target effects; seed; target; seed region ... See more keywords
Role of a “Magic” Methyl: 2′-Deoxy-2′-α-F-2′-β-C-methyl Pyrimidine Nucleotides Modulate RNA Interference Activity through Synergy with 5′-Phosphate Mimics and Mitigation of Off-Target Effects
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c01679
Abstract: Although 2′-deoxy-2′-α-F-2′-β-C-methyl (2′-F/Me) uridine nucleoside derivatives are a successful class of antiviral drugs, this modification had not been studied in oligonucleotides. Herein, we demonstrate the facile synthesis of 2′-F/Me-modified pyrimidine phosphoramidites and their subsequent incorporation… read more here.
Keywords: target effects; modification; deoxy methyl; activity ... See more keywords
Can target effects or discomfort ratings discriminate between small-arms weapon configurations?
Sign Up to like & getrecommendations! Published in 2020 at "Ergonomics"
DOI: 10.1080/00140139.2020.1834625
Abstract: Abstract Defence acquisitions use accuracy measures as a discriminating factor in weapon purchases, but assessments are generally completed in static, supported postures at static targets with few differences being seen between configurations. The aim of… read more here.
Keywords: target effects; addition mass; accuracy; ratings discriminate ... See more keywords