ExsgRNA: reduce off-target efficiency by on-target mismatched sgRNA
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac183
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing technology has been widely used to facilitate efficient genome editing. Current popular sgRNA design tools only consider the sgRNA perfectly matched to the target site and… read more here.
Keywords: exsgrna reduce; target; reduce target; efficiency target ... See more keywords
CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity
Sign Up to like & getrecommendations! Published in 2019 at "Bioinformatics"
DOI: 10.1093/bioinformatics/bty1061
Abstract: Motivation The CRISPR-Cpf1 system has been successfully applied in genome editing. However, target efficiency of the CRISPR-Cpf1 system varies among different gRNA sequences. Results In this study, we reanalyzed the published CRISPR-Cpf1 gRNAs data and… read more here.
Keywords: target efficiency; crispr cpf1; crispr;