Labour‐saving construction of a target protein interaction network by selective culture and high‐throughput sequencing
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology Journal"
DOI: 10.1002/biot.202100204
Abstract: Yeast two‐hybrid (Y2H) is a classic method of screening for protein‐protein interactions. However, the operation process is labor intensive and time consuming, and there is a high possibility of false positives and false negatives. read more here.
Keywords: protein; protein interaction; target protein; construction target ... See more keywords
BioID: A Proximity-Dependent Labeling Approach in Proteomics Study.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8814-3_10
Abstract: Biological activities are mainly executed by proteins and in most of the occasions these activities are accomplished by protein complexes or through protein-protein interactions (PPI). So it is critical to reveal how the protein complexes… read more here.
Keywords: protein; target protein; interacting partners; dependent labeling ... See more keywords
SUMO in Drosophila Development.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-50044-7_15
Abstract: The ubiquitin -like protein SUMO is conjugated covalently to hundreds of target proteins in organisms throughout the eukaryotic domain. Genetic and biochemical studies using the model organism Drosophila melanogaster are beginning to reveal many essential… read more here.
Keywords: target protein; drosophila development; sumo drosophila; biology ... See more keywords
Selective Inhibitor Design for Kinase Homologs Using Multiobjective Monte Carlo Tree Search
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Chemical Information and Modeling"
DOI: 10.1021/acs.jcim.2c00787
Abstract: Designing highly selective molecules for a drug target protein is a challenging task in drug discovery. This task can be regarded as a multiobjective problem that simultaneously satisfies criteria for various objectives, such as selectivity… read more here.
Keywords: structure; selective inhibitors; structure generator; target protein ... See more keywords
Selective purification and chemical labeling of a target protein on ruthenium photocatalyst-functionalized affinity beads.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc01595j
Abstract: Selective purification and chemical labeling of a target protein in a protein mixture were simultaneously achieved on the surface of affinity beads functionalized with ligands, such as benzenesulfonamide and methotrexate (MTX), and a ruthenium complex… read more here.
Keywords: ruthenium; affinity beads; target protein; chemical ... See more keywords
A thiol-activated fluorogenic probe for detection of a target protein.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc02029g
Abstract: A novel fluorogenic probe for facile and efficient detection of a target protein that binds to a bioactive small molecule was developed. The probe was composed of a thiol-activated fluorogenic moiety bearing an alkyne tag… read more here.
Keywords: fluorogenic probe; detection target; protein; target protein ... See more keywords
A simple toolset to identify endogenous post-translational modifications for a target protein: a snapshot of the EGFR signaling pathway
Sign Up to like & getrecommendations! Published in 2017 at "Bioscience Reports"
DOI: 10.1042/bsr20170919
Abstract: Identification of a novel post-translational modification (PTM) for a target protein, defining its physiologic role and studying its potential cross-talk with other PTMs is a challenging process. A set of highly sensitive tools termed as… read more here.
Keywords: egfr signaling; post translational; target protein; signaling pathway ... See more keywords
Crystals of TELSAM–target protein fusions that exhibit minimal crystal contacts and lack direct inter-TELSAM contacts
Sign Up to like & getrecommendations! Published in 2022 at "Open Biology"
DOI: 10.1098/rsob.210271
Abstract: While conducting pilot studies into the usefulness of fusion to TELSAM polymers as a potential protein crystallization strategy, we observed novel properties in crystals of two TELSAM–target protein fusions, as follows. (i) A TELSAM–target protein… read more here.
Keywords: target protein; telsam target; crystal contacts; target ... See more keywords
Hybrid phenotype mining method for investigating off-target protein and underlying side effects of anti-tumor immunotherapy
Sign Up to like & getrecommendations! Published in 2020 at "BMC Medical Informatics and Decision Making"
DOI: 10.1186/s12911-020-1105-4
Abstract: It is of utmost importance to investigate novel therapies for cancer, as it is a major cause of death. In recent years, immunotherapies, especially those against immune checkpoints, have been developed and brought significant improvement… read more here.
Keywords: ontology; target protein; target; hybrid phenotype ... See more keywords
Phosphorylation‐linked complex profiling identifies assemblies required for Hippo signal integration
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Systems Biology"
DOI: 10.15252/msb.202211024
Abstract: While several computational methods have been developed to predict the functional relevance of phosphorylation sites, experimental analysis of the interdependency between protein phosphorylation and Protein–Protein Interactions (PPIs) remains challenging. Here, we describe an experimental strategy… read more here.
Keywords: complex profiling; phosphorylation linked; target protein; linked complex ... See more keywords
Novel Benzimidazole-Based Compounds as Antimicrobials: Synthesis, Molecular Docking, Molecular Dynamics and in silico ADME Profile Studies.
Sign Up to like & getrecommendations! Published in 2022 at "Acta chimica Slovenica"
DOI: 10.17344/acsi.2021.7314
Abstract: Some novel benzimidazole derivatives were synthesized and their antimicrobial activities were evaluated. Compounds 3a and 3b exhibited excellent antibacterial activity with MIC values read more here.
Keywords: molecular docking; molecular dynamics; target protein; novel benzimidazole ... See more keywords