An in silico approach to identify and prioritize miRNAs target sites polymorphisms in colorectal cancer and obesity
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Medicine"
DOI: 10.1002/cam4.3546
Abstract: Colorectal cancer (CRC) and obesity are linked clinical entities with a series of complex processes being engaged in their development. MicroRNAs (miRNAs) participate in these processes through regulating CRC and obesity‐related genes. This study aimed… read more here.
Keywords: obesity; colorectal cancer; target sites; obesity crc ... See more keywords
Perspective: It is time to consider new ways to attack unpesticidable (undruggable) target sites by designing peptide pesticides.
Sign Up to like & getrecommendations! Published in 2022 at "Pest management science"
DOI: 10.1002/ps.6817
Abstract: Evolved resistance and regulatory deregistration have severely limited farmers' pesticide options. Many potential new pesticide target sites have been elucidated using targeted gene suppression and mutational tools, but few small molecules could be found that… read more here.
Keywords: consider new; perspective time; peptide pesticides; target sites ... See more keywords
Evolutionary conservation of influenza A PB2 sequences reveals potential target sites for small molecule inhibitors.
Sign Up to like & getrecommendations! Published in 2017 at "Virology"
DOI: 10.1016/j.virol.2017.06.009
Abstract: The influenza A basic polymerase protein 2 (PB2) functions as part of a heterotrimer to replicate the viral RNA genome. To investigate novel PB2 antiviral target sites, this work identified evolutionary conserved regions across the… read more here.
Keywords: evolutionary conservation; pb2; target sites; conservation influenza ... See more keywords
Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15204-0
Abstract: A number of recent papers report that standing genetic variation in natural populations includes ubiquitous polymorphisms within target sites for Cas9-based gene drive (CGD) and that these “drive resistant alleles” (DRA) preclude the successful application… read more here.
Keywords: target sites; target; natural populations; genetic variation ... See more keywords
CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-022-28244-5
Abstract: CRISPR-Cas9 genome editing has potential to cure diseases without current treatments, but therapies must be safe. Here we show that CRISPR-Cas9 editing can introduce unintended mutations in vivo, which are passed on to the next… read more here.
Keywords: structural variants; target target; crispr cas9; target ... See more keywords
Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq
Sign Up to like & getrecommendations! Published in 2021 at "Nature Protocols"
DOI: 10.1038/s41596-020-00453-6
Abstract: Digested genome sequencing (Digenome-seq) is a highly sensitive, easy-to-carry-out, cell-free method for experimentally identifying genome-wide off-target sites of programmable nucleases and deaminases (also known as base editors). Genomic DNA is digested in vitro using clustered… read more here.
Keywords: genome wide; dna; identifying genome; target sites ... See more keywords
Unlocking HDR-mediated nucleotide editing by identifying high-efficiency target sites using machine learning
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-39142-0
Abstract: Editing individual nucleotides is a crucial component for validating genomic disease association. It is currently hampered by CRISPR-Cas-mediated “base editing” being limited to certain nucleotide changes, and only achievable within a small window around CRISPR-Cas… read more here.
Keywords: hdr mediated; machine learning; using machine; hdr ... See more keywords
RGEN-seq for highly sensitive amplification-free screen of off-target sites of gene editors
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-03160-8
Abstract: Sensitive detection of off-target sites produced by gene editing nucleases is crucial for developing reliable gene therapy platforms. Although several biochemical assays for the characterization of nuclease off-target effects have been recently published, significant technical… read more here.
Keywords: amplification; detection; target; gene ... See more keywords
Regulation of human UDP-glycosyltransferase (UGT) genes by miRNAs
Sign Up to like & getrecommendations! Published in 2022 at "Drug Metabolism Reviews"
DOI: 10.1080/03602532.2022.2048846
Abstract: Abstract The human UGT gene superfamily is divided into four subfamilies (UGT1, UGT2, UGT3 and UGT8) that encodes 22 functional enzymes. UGTs are critical for the metabolism and clearance of numerous endogenous and exogenous compounds,… read more here.
Keywords: mirna target; ugt genes; regulation; target sites ... See more keywords
Optimization of siRNA delivery to target sites: issues and future directions
Sign Up to like & getrecommendations! Published in 2018 at "Expert Opinion on Drug Delivery"
DOI: 10.1080/17425247.2018.1520836
Abstract: ABSTRACT Introduction: The discovery of RNA interference (RNAi) earned the 2006 Nobel Prize in Physiology or Medicine for its biological significance and potential for developing novel therapeutics. The small interfering RNA (siRNA) is the most… read more here.
Keywords: sirna delivery; delivery target; optimization sirna; delivery ... See more keywords
The HIV-1 Capsid-Targeted Inhibitor GSK878 Alters Selection of Target Sites for HIV DNA Integration.
Sign Up to like & getrecommendations! Published in 2023 at "AIDS research and human retroviruses"
DOI: 10.1089/aid.2022.0161
Abstract: Decades of effort have yielded highly effective antiviral agents to treat HIV, but viral strains have evolved resistance to each inhibitor type, focusing attention on the importance of developing new inhibitor classes. A particularly promising… read more here.
Keywords: hiv capsid; integration; inhibitor; target sites ... See more keywords